Characterization of the oligosaccharides of plasma sex hormone binding globulin from noncirrhotic alcoholic patients☆
| Autor: | Alejandro Erices, Ximena Lioi, Luis Valladares, Iturriaga H |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Adult
Male medicine.medical_specialty Glycosylation Globulin medicine.medical_treatment Clinical Biochemistry Oligosaccharides Chemical Fractionation Chitobiose Tritium Biochemistry Chromatography Affinity Steroid chemistry.chemical_compound Endocrinology Sex hormone-binding globulin Sex Hormone-Binding Globulin Internal medicine Concanavalin A polycyclic compounds medicine Humans Liver Diseases Alcoholic Molecular Biology reproductive and urinary physiology Pharmacology chemistry.chemical_classification Dose-Response Relationship Drug biology Organic Chemistry Lectin Dihydrotestosterone Middle Aged Oligosaccharide Chromatography Agarose Wheat germ agglutinin chemistry biology.protein hormones hormone substitutes and hormone antagonists Protein Binding |
| Zdroj: | Steroids. 65:275-280 |
| ISSN: | 0039-128X |
| DOI: | 10.1016/s0039-128x(00)00086-6 |
| Popis: | In previous reports we have demonstrated high plasma levels of sex hormone-binding globulin (SHBG) in asymptomatic alcoholic men. In the present work the physicochemical properties of SHBG from plasma of noncirrhotic alcoholic patients have been further compared with SHBG of control subjects. Steroid binding to SHBG was similar for the two groups: alcoholic men, K(d) of 0.62 +/- 0.07 nM and control individuals, K(d) of 0.70 +/- 0.10 nM. The structure of oligosaccharides attached to SHBG from controls and alcoholic men were determined by using serial chromatography. Our data indicated that 7% of SHBG of control individuals was not retarded by the Con-A column, whereas approximately 30% of SHBG of alcoholic men eluted in the void volume of Con A. Approximately 46% of SHBG of alcoholics applied to Con A, possessed biantennary complex oligosaccharides, as indicated by the fact that it could be eluted with methyl-alpha-D-glucopyranoside and by its retention on wheat germ agglutinin; in contrast, when SHBG from control men was analyzed, approximately 51% was eluted with methyl-alpha-D-glucopyranoside. Approximately 9% of the biantennary complex oligosaccharides on SHBG of control men and none of those on SHBG from alcoholic men were fucosylated on the chitobiose core, as determined by chromatography on Lenn culinaris lectin. Galactosylated oligosaccharides were also present on the SHBG fraction as indicated by its interaction with Ricinus communis-I. Approximately 24% of SHBG of alcoholic men and 39% of those on SHBG from control individuals applied to Con-A were retained and could be eluted with methyl-alpha-D-mannopyranoside. Evidence based on the binding on mannoside-eluted SHBG to Con-A, wheat germ agglutinin, and R. communis-I indicated that at least the SHBG in this fraction, from alcoholics or controls, contained two glycosylation sites and that the sites were differentially glycosylated. |
| Databáze: | OpenAIRE |
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