Short-Wave Infrared Fluorescence Chemical Sensor for Detection of Otitis Media
| Autor: | David Zarabanda, Matthew Bogyo, David M. Huland, Surya Pratap Singh, Joshua J. Yim, Paola Solis-Pazmino, Martina Tholen, Zhixin Cao, Raana Kashfi-Sadabad, Anping Xia, Tulio A. Valdez |
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| Rok vydání: | 2020 |
| Předmět: |
Pathology
medicine.medical_specialty Ear Middle Bioengineering Otoscopy 02 engineering and technology 01 natural sciences Fluorescence Middle ear infection medicine Short wave infrared Humans Otoscope Child Instrumentation Overdiagnoses Fluid Flow and Transfer Processes business.industry Otitis Media with Effusion Process Chemistry and Technology 010401 analytical chemistry 021001 nanoscience & nanotechnology Chemical sensor 0104 chemical sciences Otitis Media medicine.anatomical_structure Otitis Child Preschool Middle ear medicine.symptom 0210 nano-technology business |
| Zdroj: | ACS sensors. 5(11) |
| ISSN: | 2379-3694 |
| Popis: | Otitis media (OM) or middle ear infection is one of the most common diseases in young children around the world. The diagnosis of OM is currently performed using an otoscope to detect middle ear fluid and inflammatory changes manifested in the tympanic membrane. However, conventional otoscopy cannot visualize across the tympanic membrane or sample middle ear fluid. This can lead to low diagnostic certainty and overdiagnoses of OM. To improve the diagnosis of OM, we have developed a short-wave infrared (SWIR) otoscope in combination with a protease-cleavable biosensor, 6QC-ICG, which can facilitate the detection of inflammatory proteases in the middle ear with an increase in contrast. 6QC-ICG is a fluorescently quenched probe, which is activated in the presence of cysteine cathepsin proteases that are up-regulated in inflammatory immune cells. Using a preclinical model and custom-built SWIR otomicroscope in this proof-of-concept study, we successfully demonstrated the feasibility of robustly distinguishing inflamed ears from controls (p = 0.0006). The inflamed ears showed an overall signal-to-background ratio of 2.0 with a mean fluorescence of 81 ± 17 AU, while the control ear exhibited a mean fluorescence of 41 ± 11 AU. We envision that these fluorescently quenched probes in conjunction with SWIR imaging tools have the potential to be used as an alternate/adjunct tool for objective diagnosis of OM. |
| Databáze: | OpenAIRE |
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