Antibodies to maitotoxin elicited by immunization with toxin-fragment conjugates
| Autor: | Gary S. Bignami, David F. Waller, Takeshi Yasumoto |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Ciguatera
Toxicology medicine.disease_cause Binding Competitive Mice chemistry.chemical_compound medicine Animals Maitotoxin biology Toxin Immunotoxins Oxocins Serum Albumin Bovine Anatomy medicine.disease biology.organism_classification Molecular biology Peptide Fragments Gambierdiscus toxicus chemistry Polyclonal antibodies Immunoglobulin G Hemocyanins biology.protein Immunization Marine Toxins Antitoxins Antibody Carrier Proteins Marine toxin Keyhole limpet hemocyanin |
| Zdroj: | Toxicon. 34:1393-1397 |
| ISSN: | 0041-0101 |
| DOI: | 10.1016/s0041-0101(96)00094-3 |
| Popis: | G. S. Bignami, D. F. Waller and T. Yasumoto. Antibodies to maitotoxin elicited by immunization with toxin-fragment conjugates. Toxicon 34, 1393-1397, 1996.-Maitotoxin is a water-soluble polyether toxin produced by the benthic dinoflagellate, Gambierdiscus toxicus. Toxin fragments generated by periodate oxidation were conjugated to keyhole limpet hemocyanin, and the resulting immunogens were used to elicit maitotoxin-specific antibodies in mice. A competitive immunoassay developed with polyclonal IgG antibodies detected ∼3 nM purified maitotoxin standard (IC 50 ∼ 13 nM; 45 ng/ml) but did not detect other polyether marine toxins. These are the first reported maitotoxin-specific antibodies. |
| Databáze: | OpenAIRE |
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