Micronuclear and sister chromatid exchange analyses in peripheral lymphocytes of patients with oral lichen planus - a pilot study
| Autor: | Alp Saruhanoglu, Kivanc Cefle, B Sevinç, Sukru Palanduz, Nilgün Duman, Saman Warnakulasuriya, H Tanyeri, Sevda Ozel, Savas Ozturk, Sertan Ergun |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male medicine.medical_specialty Saliva Pathology Pilot Projects Sister chromatid exchange Biology Malignancy Gastroenterology stomatognathic system Internal medicine Biomarkers Tumor medicine Humans Lymphocyte Count Lymphocytes Family history General Dentistry Micronuclei Chromosome-Defective Dental Plaque Index Cancer Middle Aged Prognosis medicine.disease stomatognathic diseases Otorhinolaryngology Case-Control Studies Micronucleus test Cohort Female Oral lichen planus Periodontal Index Secretory Rate Sister Chromatid Exchange Lichen Planus Oral |
| Zdroj: | Oral Diseases. 15:499-504 |
| ISSN: | 1601-0825 1354-523X |
| DOI: | 10.1111/j.1601-0825.2009.01576.x |
| Popis: | Objective: The purpose of this study was to determine the genetic instability of peripheral blood lymphocytes from patients diagnosed with oral lichen planus (OLP) by investigation of frequencies of micronuclei (MN) and sister chromatid exchange (SCE). Materials and Methods: A total of 22 newly diagnosed and untreated patients with OLP of same severity scores and twenty healthy controls participated in this study. They were all non-smokers with no previous history or family history of cancer. The periodontal status, flow rate and buffering capacity of whole mouth saliva were recorded. SCE and MN analyses were performed on peripheral blood lymphocytes of OLP patients and healthy controls. Results: The frequencies of MN (50.00 ± 22.36) and SCE (6.89 ± 1.48) in OLP patients were found to be significantly elevated compared with that in normal individuals (25.20 ± 9.52 and 5.93 ± 1.31; z = 3.946, P = 0.0001; z = 2.346, P = 0.019). There were no significant differences in the MN frequency and SCE between the two subgroups with reticular or erosive types of OLP. Conclusion: These pilot data indicate an increased genomic instability in peripheral blood lymphocytes of a cohort of Turkish patients diagnosed with oral lichen planus as compared with that of healthy individuals. As patients with OLP may have an increased or potential risk for oral malignancy, these assays could be used in translational research to monitor beneficial effects of interventions and long-term prognosis. |
| Databáze: | OpenAIRE |
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