The optimal ethanol lock therapy regimen for treatment of biofilm-associated catheter infections: an in-vitro study
| Autor: | C. Rodríguez, M. J. Pérez-Granda, B. Alonso, María Guembe, Emilio Bouza, A. Rodríguez-Huerta |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Catheters medicine.drug_class 030106 microbiology Antibiotics Tetrazolium Salts Pharmacology medicine.disease_cause Enterococcus faecalis 03 medical and health sciences Staphylococcus epidermidis medicine Humans Candida albicans Candida Infection Control Bacteria Ethanol Staining and Labeling biology Heparin business.industry Biofilm General Medicine biology.organism_classification Regimen Metabolism Infectious Diseases Staphylococcus aureus Biofilms Catheter-Related Infections business Disinfectants medicine.drug |
| Zdroj: | Journal of Hospital Infection. 100:e187-e195 |
| ISSN: | 0195-6701 |
| DOI: | 10.1016/j.jhin.2018.04.007 |
| Popis: | Ethanol-based lock therapy (LT) solutions are used as an alternative to antibiotics for the conservative management of catheter-related bloodstream infection. However, no clear consensus on regimen or dose has been reached.To find the ethanol-based lock solution containing a sufficiently low concentration of ethanol for reduction of the metabolic activity of bacterial and fungal biofilms.Using an in-vitro model, three concentrations of ethanol (25%, 40%, 70%) were tested, with and without 60 IU of heparin, at six different time-points and against 24 h preformed biofilms of Staphylococcus aureus ATCC29213, Staphylococcus epidermidis (clinical isolate), Enterococcus faecalis ATCC33186, Candida albicans ATCC14058, and Escherichia coli ATCC25922. The reduction in the metabolic activity of the biofilm was measured using the tetrazolium salt assay and LT was considered to be successful when metabolic activity fell by90%. Regrowth inhibition was then tested within 24 h and seven days after each LT regimen only at the ethanol concentration of the most successful LT regimen.The most successful LT was achieved with 40% ethanol + 60 IU of heparin only at 24, 72, and 24 h for seven-day regimens (P 0.05). However, none of the regimens reached 45% RI within seven days of therapy.According to our in-vitro data, an ethanol-based lock solution with 40% ethanol + 60 IU heparin administered daily for 72 h is sufficient to almost eradicate the metabolic activity of bacterial and fungal biofilms. Future studies are needed to study cell regrowth after LT. |
| Databáze: | OpenAIRE |
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