Human Genital Melanocytes as Androgen Target Cells
| Autor: | Susumu Takayasu, Hiroto Terashi, Taketsugu Tadokoro, Satoshi Itami, Ko Hosokawa |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Cholestenone 5 alpha-Reductase
medicine.medical_specialty 17-Hydroxysteroid Dehydrogenases Transcription Genetic medicine.drug_class Tyrosinase hormone Gene Expression Human skin Dermatology tyrosinase Biology Melanocyte urologic and male genital diseases Polymerase Chain Reaction Biochemistry chemistry.chemical_compound androgen receptor Internal medicine medicine Humans Genitalia RNA Messenger Receptor Molecular Biology 5a-reductase Cell Nucleus Monophenol Monooxygenase Cyproterone acetate Cell Biology Blotting Northern Androgen Immunohistochemistry Isoenzymes Androgen receptor Endocrinology medicine.anatomical_structure chemistry Receptors Androgen Dihydrotestosterone Androgens Melanocytes Oxidoreductases medicine.drug |
| Zdroj: | Journal of Investigative Dermatology. 109(4):513-517 |
| ISSN: | 0022-202X |
| DOI: | 10.1111/1523-1747.ep12336630 |
| Popis: | As some parts of human skin - such as genital and areolar skin - become pigmented after puberty, melanocytes in these regions are thought to be sex hormone target cells. We immunohistochemically localized androgen receptors in the nuclei of cultured human genital melanocytes by monoclonal and polyclonal antibodies. When these cells were incubated with [1,2-3H]-testosterone, the major metabolite in the medium was dihydrotestosterone and 5alpha-reduction predominated over 17beta-oxidation. Androgen receptor and type I 5alpha-reductase mRNAs could be detected in genital melanocytes by reverse transcriptase polymerase chain reaction. The tyrosinase activity was stimulated by the addition of androgen. This stimulation was antagonized by cyproterone acetate, whereas tyrosinase mRNA expression was not affected by androgen. These results indicate that human genital melanocytes are androgen target cells, and that androgen plays a role for pigmentation in the specific regional skin after puberty. |
| Databáze: | OpenAIRE |
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