Indocyanine Green J Aggregates in Polymersomes for Near-Infrared Photoacoustic Imaging
| Autor: | Konstantin V Sokolov, Sangheon Han, Ehsan Moaseri, Federica Scaletti, Rosalie Caplan, Alice Cao, Richard R. Bouchard, Lauren Truong, Thomas M. Truskett, Behzad Changalvaie, Keith P. Johnston |
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| Rok vydání: | 2019 |
| Předmět: |
Indocyanine Green
Materials science genetic structures Contrast Media Mice Nude Breast Neoplasms 02 engineering and technology 010402 general chemistry 01 natural sciences Nanocapsules Polyethylene Glycols Absorbance Photoacoustic Techniques chemistry.chemical_compound Mice Cell Line Tumor Animals Humans General Materials Science J-aggregate Polyglactin 910 Ovarian Neoplasms Polyethylenimine Water 021001 nanoscience & nanotechnology 0104 chemical sciences Molecular Imaging Monomer chemistry Polymersome Biophysics Emulsions Female 0210 nano-technology Indocyanine green Ethylene glycol Oils |
| Zdroj: | ACS applied materialsinterfaces. 11(50) |
| ISSN: | 1944-8252 |
| Popis: | Clinical translation of photoacoustic imaging (PAI) has been limited by the lack of near-infrared (NIR) contrast agents with low toxicity required for regulatory approval. Herein, J aggregates of indocyanine green (ICG) with strong NIR absorbance were encapsulated at high loadings within small 77 nm polymersomes (nanocapsules) composed of poly(lactide-co-glycolide-b-poly(ethylene glycol)) (PLGA-b-PEG) bilayers, thus enabling PAI of of breast and ovarian cancer cells with high specificity and a sensitivity at the level of ∼100 total cells. All of the major components of the polymersomes are FDA approved and used in the clinic. During formation of polymersomes with a water-in-oil-in-water double emulsion process, loss of ICG from the ICG J aggregates was minimized by coating them with a layer of branched polyethylenimine and by providing excess "sacrificial" ICG to adsorb at the oil-water interfaces. The encapsulated J aggregates were protected against dissociation by the polymersome shell for 24 h in 100% fetal bovine serum, after which the polymersomes biodegraded and the J aggregates dissociated to ICG monomers. |
| Databáze: | OpenAIRE |
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