Plant-made salmonella bacteriocins salmocins for control of salmonella pathovars
| Autor: | Tobias Schneider, Anett Stephan, Steve Schulz, Marcel Naumann, Simone Hahn-Löbmann, Martin Kleinschmidt, Daniel Tuse, Anatoli Giritch, Yuri Gleba |
|---|---|
| Přispěvatelé: | Publica |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Serotype Salmonella 030106 microbiology lcsh:Medicine Gene Expression Microbial Sensitivity Tests medicine.disease_cause Article Microbiology 03 medical and health sciences Bacterial Proteins Bacteriocins Bacteriocin medicine Data Mining Cloning Molecular lcsh:Science Escherichia coli Multidisciplinary biology business.industry lcsh:R Computational Biology Salmonella enterica Plants Genetically Modified biology.organism_classification Food safety Antimicrobial Recombinant Proteins United States 030104 developmental biology Colicin Food Microbiology Food Preservatives lcsh:Q business Genome Bacterial |
| Zdroj: | Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-10 (2018) |
| Popis: | Salmonella enterica causes an estimated 1 million illnesses in the United States each year, resulting in 19,000 hospitalizations and 380 deaths, and is one of the four major global causes of diarrhoeal diseases. No effective treatments are available to the food industry. Much attention has been given to colicins, natural non-antibiotic proteins of the bacteriocin class, to control the related pathogen Escherichia coli. We searched Salmonella genomic databases for colicin analogues and cloned and expressed in plants five such proteins, which we call salmocins. Among those, SalE1a and SalE1b were found to possess broad antimicrobial activity against all 99 major Salmonella pathovars. Each of the two salmocins also showed remarkably high potency (>106 AU/µg recombinant protein, or >103 higher than colicins) against major pathogenic target strains. Treatment of poultry meat matrices contaminated with seven key pathogenic serovars confirmed salmocin efficacy as a food safety intervention against Salmonella. |
| Databáze: | OpenAIRE |
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