Adrenoceptor Expression during Intervertebral Disc Degeneration

Autor: C. Fleege, Richard Bostelmann, Andrea Meurer, Frank Zaucke, Karima El Bagdadi, Johannes Kupka, M. Rickert, Daniel W. Chan, Marco Brenneis, Zsuzsa Jenei-Lanzl, Annika Kohler
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Male
0301 basic medicine
Decorin
Stimulation
Intervertebral Disc Degeneration
lcsh:Chemistry
Extracellular matrix
Mice
0302 clinical medicine
sympathicus
Gene expression
lcsh:QH301-705.5
Spectroscopy
Mitogen-Activated Protein Kinase 1
IVD degeneration (IVDD)
Mitogen-Activated Protein Kinase 3
biology
Chemistry
General Medicine
musculoskeletal system
Receptors
Adrenergic
Computer Science Applications
Cell biology
medicine.anatomical_structure
Female
Intracellular
musculoskeletal diseases
Adrenergic receptor
MAP Kinase Signaling System
Mice
Transgenic
adrenoceptors
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
medicine
Animals
Humans
ddc:610
Physical and Theoretical Chemistry
Molecular Biology
Aged
Cartilage oligomeric matrix protein
intervertebral disc (IVD)
Organic Chemistry
Intervertebral disc
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Gene Expression Regulation
biology.protein
030217 neurology & neurosurgery
Zdroj: International Journal of Molecular Sciences
Volume 21
Issue 6
International Journal of Molecular Sciences, Vol 21, Iss 6, p 2085 (2020)
ISSN: 1422-0067
DOI: 10.3390/ijms21062085
Popis: Healthy and degenerating intervertebral discs (IVDs) are innervated by sympathetic nerves, however, adrenoceptor (AR) expression and functionality have never been investigated systematically. Therefore, AR gene expression was analyzed in both tissue and isolated cells from degenerated human IVDs. Furthermore, human IVD samples and spine sections of wildtype mice (WT) and of a mouse line that develops spontaneous IVD degeneration (IVDD, in SM/J mice) were stained for ARs and extracellular matrix (ECM) components. In IVD homogenates and cells &alpha
1a-, &alpha
1b-, &alpha
2a-, &alpha
2b-, &alpha
2c-, &beta
1-, and &beta
2-AR genes were expressed. In human sections, &beta
2-AR was detectable, and its localization parallels with ECM alterations. Similarly, in IVDs of WT mice, only &beta
2-AR was expressed, and in IVDs of SM/J mice, &beta
2AR expression was stronger accompanied by increased collagen II, collagen XII, decorin as well as decreased cartilage oligomeric matrix protein expression. In addition, norepinephrine stimulation of isolated human IVD cells induced intracellular signaling via ERK1/2 and PKA. For the first time, the existence and functionality of ARs were demonstrated in IVD tissue samples, suggesting that the sympathicus might play a role in IVDD. Further studies will address relevant cellular mechanisms and thereby help to develop novel therapeutic options for IVDD.
Databáze: OpenAIRE
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