Milk and Meat Allergens from Bos taurus β-Lactoglobulin, α-Casein, and Bovine Serum Albumin: An In-Vivo Study of the Immune Response in Mice
| Autor: | Dagmara Złotkowska, Barbara Wróblewska, Ewa Fuc |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
cross-reactivity Lymphocyte mice allergy model Lactoglobulins medicine.disease_cause Immunoglobulin E Cross-reactivity immune response Feces Mice fluids and secretions 0302 clinical medicine Allergen Lymphocytes Bovine serum albumin Nutrition and Dietetics biology Chemistry food and beverages Caseins Yogurt beef medicine.anatomical_structure Milk Female Antibody lcsh:Nutrition. Foods and food supply Food Hypersensitivity Meat cow milk lcsh:TX341-641 Article Andrology 03 medical and health sciences Immune system Food allergy medicine Animals Humans Serum Albumin Allergens medicine.disease Immunity Humoral 030104 developmental biology biology.protein Cattle Spleen 030215 immunology Food Science |
| Zdroj: | Nutrients Nutrients, Vol 11, Iss 9, p 2095 (2019) Volume 11 Issue 9 |
| ISSN: | 2072-6643 |
| Popis: | The mechanism of food allergy may vary. This study aimed to compare the effects of milk, yogurt, or beef meat supplementation on humoral and cellular immune responses in a mice model. Mice were divided into four groups: The &ldquo Milk group&rdquo was sensitized with a &beta lactoglobulin (&beta lg)/&alpha casein (&alpha CN) mixture and supplemented cow milk the &ldquo Yogurt group&rdquo was sensitized with &beta lg/&alpha CN and supplemented yogurt Beef group&rdquo was immunized with bovine serum albumin (BSA) and supplemented beef meat and the &ldquo PBS group&rdquo received PBS in all procedures. ELISA was used to measure humoral response, including: Total IgE, specific IgG, and IgA. Cellular response was determined by phenotyping lymphocyte from lymphoid tissue and measuring the Th1/Th2 cytokine concentration with flow cytometry. The qPCR method was used for quantification of the fecal microbiota. The results obtained revealed a lower IgE level for the Yogurt group than for the Milk one. In the Yogurt group, the contribution of regulatory T cells to MLN and PP was higher compared to the other groups. We confirmed that diet supplementation with yogurt modulates the immune response to the prime allergen, and changes the activity of serum antibodies to milk proteins and BSA. Based on a specific antibodies level, we cannot exclude the possibility of CMA mice reaction against BSA. |
| Databáze: | OpenAIRE |
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