Characterization of Yersinia pestis Phage Lytic Activity in Human Whole Blood for the Selection of Efficient Therapeutic Phages
| Autor: | Avital Tidhar, Ida Steinberger-Levy, Sarit Moses, Moshe Aftalion, Yaron Vagima, Shahar Rotem, Emanuelle Mamroud |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Lysis phage selection Phage therapy Yersinia pestis medicine.medical_treatment viruses 030106 microbiology lcsh:QR1-502 medicine.disease_cause Article lcsh:Microbiology Microbiology Bacteriophage 03 medical and health sciences chemistry.chemical_compound Bacteriolysis bacteriophage Virology medicine Humans Bacteriophages Phage Therapy Precision Medicine human whole blood Plague biology Pathogenic bacteria Viral Load biology.organism_classification 030104 developmental biology Infectious Diseases chemistry Lytic cycle Brain heart infusion personalized phage therapy Bacteria |
| Zdroj: | Viruses, Vol 13, Iss 89, p 89 (2021) Viruses Volume 13 Issue 1 |
| ISSN: | 1999-4915 |
| Popis: | The global increase in multidrug-resistant (MDR) pathogenic bacteria has led to growing interest in bacteriophage (&ldquo phage&rdquo ) therapy. Therapeutic phages are usually selected based on their ability to infect and lyse target bacteria, using in vitro assays. In these assays, phage infection is determined using target bacteria grown in standard commercial rich media, while evaluation of the actual therapeutic activity requires the presence of human blood. In the present work, we characterized the ability of two different Yersinia pestis lytic phages (ϕA1122 and PST) to infect and kill a luminescent Y. pestis EV76 strain suspended in Brain Heart Infusion (BHI)-rich medium or in human whole blood, simulating the host environment. We found that the ability of the phages to infect and lyse blood-suspended Y. pestis was not correlated with their ability to infect and lyse BHI-suspended bacteria. While the two different phages exhibited efficient infective capacity in a BHI-suspended culture, only the PST phage showed efficient lysis ability against blood-suspended bacteria. Therefore, we recommend that for personalized phage therapy, selection of phage(s) for efficient treatment of patients suffering from MDR bacterial infections should include prior testing of the candidate phage(s) for their lysis ability in the presence of human blood. |
| Databáze: | OpenAIRE |
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