In vitro protein synthesis is affected by the herbicide 2,4-dichlorophenoxyacetic acid in Azospirillum brasilense
| Autor: | Adriana Fabra, Héctor Balegno, Gladys Mori, Viviana Rivarola |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
DNA
Bacterial 2 4-Dichlorophenoxyacetic acid DNA synthesis Cell growth Phenylalanine Azospirillum brasilense Biology Toxicology biology.organism_classification RNA Bacterial chemistry.chemical_compound Bacterial Proteins chemistry Biochemistry Leucine Protein biosynthesis 2 4-Dichlorophenoxyacetic Acid Polyamine Ribosomes Uridine Cell Division DNA Bacteria Thymidine |
| Zdroj: | Toxicology. 73:71-79 |
| ISSN: | 0300-483X |
| DOI: | 10.1016/0300-483x(92)90171-a |
| Popis: | The effects of 2,4-dichlorophenoxyacetic acid (2,4-D) on growth and protein, DNA and RNA synthesis of Azospirillum brasilense Cd were studied. At a concentration of 1 mM, 2,4-D inhibited cell growth, an effect that was reversed either by transferring bacteria to a control (2,4-D-free) medium or to a 2,4-D-treated medium supplemented with polyamines. The herbicide also affected in vitro protein synthesis, either when Azospirillum brasilense Cd's own cellular mRNA or an artificial mRNA was used. This effect was also reversed by the addition of polyamines to the 2,4-D-treated medium. Similar results were observed when DNA synthesis was studied in synchronous cultures. Taking into account the effects of this herbicide on animal cells (V.A. Rivarola and H.F. Balegno, Toxicology, 68 (1991) 109) we postulate that the mechanism of action of 2,4-D is similar on both procaryotic and eucaryotic cells, probably acting through the polyamine metabolism. |
| Databáze: | OpenAIRE |
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