THE PREPARATION AND TESTING OF ANTIHUMAN LYMPHOBLAST GLOBULIN FOR CLINICAL USE
| Autor: | D. Thomas, Whitaker Am, A. J. Woiwod, A. W. Phillips, J. S. Courtenay, Woodrooffe Jg |
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| Rok vydání: | 1976 |
| Předmět: |
Graft Rejection
medicine.medical_specialty Globulin Lymphoid Tissue Injections Subcutaneous medicine.medical_treatment Kidney Glomerulus Pharmacology Basement Membrane Cell Line Bone Marrow In vivo Internal medicine medicine Animals Humans Transplantation Homologous Horses Antilymphocyte Serum Immunosuppression Therapy Transplantation Hematology biology business.industry Glomerular basement membrane Lymphoblast Haplorhini Organ Size Skin Transplantation medicine.anatomical_structure Immunoglobulin G Injections Intravenous Toxicity biology.protein Macaca Skin grafting Immunization Antibody business Spleen |
| Zdroj: | Transplantation. 22:167-175 |
| ISSN: | 0041-1337 |
| DOI: | 10.1097/00007890-197608000-00012 |
| Popis: | Antibodies to cultured human lymphoblasts were raised in horses using a schedule employing both subcutaneous and intravenous routes of injection. Plasma from groups of horses was pooled and the IgG prepared from each pool was tested extensively for safety and immunosuppressive efficacy in vitro and in vivo. On the basis of the results of skin grafting in monkeys, only globulins derived from the first main bleeds were blended to produce a bulk for clinical use. One early pool of globulin was discarded because when undiluted, it was lethal in monkeys by the intravenous route, and another pool was discarded because it contained antibodies which bound to the glomerular basement membrane of rats. No signs of toxicity as judged by haematology, blood biochemistry, and histology were detected in monkeys receiving the clinical blend of globulin either subcutaneously or intravenously. |
| Databáze: | OpenAIRE |
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