The application of multiple reaction monitoring to assess ApoA-I methionine oxidations in diabetes and cardiovascular disease
| Autor: | Peter D. Reaven, Randall W. Nelson, Hussein N. Yassine, Angela M. Jackson, Christoph H. Borchers, Serrine S. Lau, Dobrin Nedelkov |
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| Rok vydání: | 2014 |
| Předmět: |
Multiple reaction monitoring
Proteomics medicine.medical_specialty HDL Apolipoprotein B Peptide Oxidative phosphorylation 030204 cardiovascular system & hematology Bioinformatics Biochemistry Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Diabetes mellitus Internal medicine medicine lcsh:QH301-705.5 030304 developmental biology chemistry.chemical_classification lcsh:R5-920 0303 health sciences Methionine biology business.industry Diabetes Selected reaction monitoring Cardiovascular disease medicine.disease Mass spectrometric In vitro 3. Good health Endocrinology lcsh:Biology (General) chemistry biology.protein lcsh:Medicine (General) business |
| Zdroj: | Translational Proteomics, Vol 4, Iss C, Pp 18-24 (2014) |
| ISSN: | 2212-9626 |
| Popis: | The oxidative modification of apolipoprotein A-I’s methionine148 (M148) is associated with defective HDL function in vitro. Multiple reaction monitoring (MRM) is a mass spectrometric technique that can be used to quantitate post-translational modifications. In this study, we developed an MRM assay to monitor the abundance ratio of the peptide containing oxidized M148 to the native peptide in ApoA-I. Measurement of the oxidized-to-unoxidized-M148 ratio was reproducible (CV < 5%). The extent of methionine M148 oxidation in the HDL of healthy controls, and type 2 diabetic participants with and without prior cardiovascular events (CVD) were then examined. The results suggest a significant increase in the relative ratio of the peptide containing oxidized M148 to the unmodified peptide in the HDL of participants with diabetes and CVD (p < 0.001), compared to participants without CVD. Monitoring the abundance ratio of the peptides containing oxidized and unoxidized M148 by MRM provides a means of examining the relationship between M148 oxidation and vascular complications in CVD. |
| Databáze: | OpenAIRE |
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