Transforming growth factor beta 2 (TGFbeta 2) produces effective pleurodesis in sheep with no systemic complications
| Autor: | Kirk B. Lane, D S Ayo, Philip J. Thompson, R E Parker, R W Diters, J T Rogers, Richard W. Light, Y C G Lee |
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| Rok vydání: | 2000 |
| Předmět: |
Pulmonary and Respiratory Medicine
medicine.medical_specialty Pleural effusion medicine.medical_treatment Gastroenterology Transforming Growth Factor beta2 Transforming Growth Factor beta Fibrosis Internal medicine Biopsy medicine Animals Pleurodesis Sheep Dose-Response Relationship Drug medicine.diagnostic_test business.industry Original Articles medicine.disease Recombinant Proteins Surgery Pleural Effusion Chest tube Disease Models Animal Dose–response relationship Models Animal Toxicity Pleura business Transforming growth factor |
| Zdroj: | Thorax. 55:1058-1062 |
| ISSN: | 0040-6376 |
| DOI: | 10.1136/thorax.55.12.1058 |
| Popis: | We have recently shown that transforming growth factor (TGF)beta(2) induces effective pleurodesis in rabbits. However, rabbits have a thin pleura while humans have a thick visceral pleura. The effect of intrapleural administration of TGF beta(2) in animals with a thick pleura and its associated systemic effects have not been investigated. This study was undertaken (1) to develop a new animal model for the study of pleurodesis using sheep which have a thick pleura resembling that of humans; (2) to study the efficacy of TGF beta(2) as a pleurodesis agent in the sheep model; and (3) to assess whether histological changes occur in extrapulmonary organs after intrapleural administration of TGF beta(2).Twelve sheep were divided into four groups and were given a single intrapleural injection of TGF beta(2) in a concentration of 1.0 microg/kg, 0.5 microg/kg, 0.25 microg/kg or 0.125 microg/kg to the right pleural cavity via a chest tube. The left pleural cavity served as the control. Any pleural fluid that accumulated after the intrapleural TGF beta(2) injection was collected and analysed. The degree of pleurodesis was graded from 1 (no adhesions) to 8 (complete symphysis50% of chest wall) at day 14 when the sheep were killed. Biopsy specimens were taken from the lungs and extrapulmonary organs.All sheep that receivedor = 0.25 microg/kg TGF beta(2) developed excellent pleurodesis (score = 8) while those that received 0.125 microg/kg had a median score of 6. The pleurodesis score did not exceed 2 in the control (left) side of any sheep. Sheep receivingor = 0.50 microg/kg TGF beta(2) developed large exudative pleural effusions while those receiving a lower dose did not. The production of effusions neither hindered nor was necessary for inducing pleurodesis. There were no significant fibrotic changes in any of the extrapulmonary organs.Intrapleural injection of 0.25-1.0 microg/kg TGF beta(2) produces excellent pleurodesis in a new sheep model with no evidence of extrapulmonary fibrosis. |
| Databáze: | OpenAIRE |
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