Inhibition of liver mitochondrial membrane permeability transition pore opening by quercetin and vitamin E in streptozotocin-induced diabetic rats

Autor: Akinwunmi O. Adeoye, Oluwatoyin O. Daniel, Olufunso O. Olorunsogo, John Ojowu
Rok vydání: 2018
Předmět:
0301 basic medicine
Male
Time Factors
medicine.medical_treatment
Administration
Oral
Apoptosis
Mitochondria
Liver
medicine.disease_cause
Biochemistry
Mitochondrial Membrane Transport Proteins
Antioxidants
Glibenclamide
chemistry.chemical_compound
0302 clinical medicine
Glyburide
Vitamin E
Alanine
Liver
030220 oncology & carcinogenesis
Mitochondrial Membranes
Quercetin
medicine.drug
medicine.medical_specialty
Biophysics
Permeability
Streptozocin
Diabetes Mellitus
Experimental
03 medical and health sciences
Diabetes mellitus
Internal medicine
medicine
Animals
Rats
Wistar
Molecular Biology
Cholesterol
Mitochondrial Permeability Transition Pore
Cell Biology
medicine.disease
Streptozotocin
Rats
Oxidative Stress
030104 developmental biology
Endocrinology
Glucose
chemistry
Lipid Peroxidation
Mitochondrial Swelling
Oxidative stress
Zdroj: Biochemical and biophysical research communications. 504(2)
ISSN: 1090-2104
Popis: Diabetes mellitus is a chronic metabolic disorder characterized by rise in blood glucose levels and generation of free radicals which could induce mitochondrial membrane permeability transition (MMPT) pore opening. This study examined the in vivo action of quercetin and vitamin E on MMPT in the liver of streptozotocin-induced diabetic rats orally pre-treated with 30 mg quercetin/kg body weight (STZQ), 10 mg vitamin E/kg body weight (STZVit.E) and 0.6 mg glibenclamide/kg body weight (STZG). Male albino wistar rats were used in the study and were injected intraperitonially with streptozotocin (STZ) (45 mg/kg body weight) in citrate buffer. The degrees of serum and tissue peroxidation, alanine and aspartate aminotransferase activity were investigated. MMPT pore was assessed as mitochondrial swelling and was monitored spectrophotometrically as changes in absorbance at 540 nm under succinate-energized condition. There was significant increase in serum glucose level, degree of tissue peroxidation, alanine and aspartate aminotransferase activity, cholesterol and triglycerides values in the diabetic control rats following streptozotocin induction. All the treatment had effect on the damage caused by streptozotocin. Quercetin exhibited the highest chemopreventive activity. Quercetin and vitamin E significantly reduced the blood glucose level, degree of tissue peroxidation and alanine and aspartate amino transferase activity. In vivo rat liver MMPT pore was opened in diabetic control rats and significantly inhibited in STZQ, STZV and STZG treated groups by 72.3%, 58.5% and 87.5% respectively. The activity of quercetin and vitamin E were substantiated by histopathological evaluation. Our study suggests that quercetin is an effective therapeutic agent for preventing hepatic tissues from oxidative stress resulting from streptozotocin-induced diabetes by inhibiting apoptotic processes in their target cells.
Databáze: OpenAIRE
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