Drospirenone intake alters plasmatic steroid levels and cyp17a1 expression in gonads of juvenile sea bass
| Autor: | Denise Fernandes, Maria Blanco, Mercedes Blázquez, Cinta Porte, Paula Medina |
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| Přispěvatelé: | Fundação para a Ciência e a Tecnologia (Portugal), Comisión Interministerial de Ciencia y Tecnología, CICYT (España) |
| Rok vydání: | 2016 |
| Předmět: |
Fish Proteins
0301 basic medicine medicine.medical_specialty food.ingredient Health Toxicology and Mutagenesis Sea bass 010501 environmental sciences Toxicology 01 natural sciences 03 medical and health sciences Bass (fish) food Contraceptive Agents Internal medicine medicine Animals Androstenedione Gonadal Steroid Hormones Gonads Testosterone Mineralocorticoid Receptor Antagonists 0105 earth and related environmental sciences biology fungi Drospirenone General Medicine biology.organism_classification Pollution cyp17a1 Metabolism 030104 developmental biology Endocrinology Sex steroids CYP17A1 Pregnenolone Androstenes Bass Female Dicentrarchus Progestins medicine.drug |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0269-7491 |
| DOI: | 10.1016/j.envpol.2016.03.007 |
| Popis: | 8 pages, 4 figures, 3 tables Drospirenone (DRO) is one of the most widely used progestins in contraceptive treatments and hormone replacement therapies. The pharmacokinetics and potential toxicological effects of DRO were investigated in juvenile sea bass (Dicentrarchus labrax) exposed through the diet (0.01–10 μg DRO/g) for up to 31 days. DRO was detected in the blood (4–27 ng/mL) of fish exposed to the highest concentration, with no significant bioaccumulation over time and no alteration of hepatic metabolizing enzymes, namely, CYP1A and CYP3A-catalysed activities and UDP-glucuronyltransferase (UGT). Pregnenolone (P5), progesterone (P4), 17α-hydroxyprogesterone (17P4), 17α-hydroxypregnenolone (17P5), androstenedione (AD) and testosterone (T) were determined in plasma and gene expression of cyp17a1, cyp19a1a and cyp11β analysed by qRT-PCR in gonads. The significant increase in plasmatic levels of 17P5, 17P4 and AD detected after 31 days exposure to 10 ng DRO/g together with the increased expression of cyp17a1 in females evidence the ability of DRO to alter steroid synthesis at low intake concentrations (7 ng DRO/day). However, the potential consequences of this steroid shift for female reproduction remain to be investigated Maria Blanco acknowledges a pre-doctoral fellowship (FPI, BES-2012-054438) from the Spanish Government and Paula Medina the pre-doctoral fellowship MECE2, ANT0806. Denise Fernandes acknowledges a post-doctoral fellowship (SFRH/BPD/34289/2006) from the Portuguese Fundação para a Ciência e Tecnologia (FCT) of the Ministry of Science and Technology of Portugal. This study was supported by the Spanish National Plan of Research (Project Ref. CGL2011-24538) |
| Databáze: | OpenAIRE |
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