Identification of Bacillus thuringiensis Cry1AbMod binding-proteins from Spodoptera frugiperda
| Autor: | Isabel Gómez, Blanca I. García-Gómez, Diana L. Martínez de Castro, Mario Soberón, Alejandra Bravo |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Physiology Genetically modified crops CD13 Antigens Spodoptera medicine.disease_cause Biochemistry DNA-binding protein Aminopeptidase Microbiology Hemolysin Proteins 03 medical and health sciences Cellular and Molecular Neuroscience Endocrinology Bacterial Proteins Bacillus thuringiensis medicine Animals Peptide sequence Bacillus thuringiensis Toxins Microvilli 030102 biochemistry & molecular biology biology Toxin fungi biology.organism_classification Actins Recombinant Proteins Endotoxins 030104 developmental biology Cry1Ac Insect Proteins |
| Zdroj: | Peptides. 98:99-105 |
| ISSN: | 0196-9781 |
| DOI: | 10.1016/j.peptides.2017.09.013 |
| Popis: | Bacillus thuringiensis Cry toxins are currently used for pest control in transgenic crops but evolution of resistance by the insect pests threatens the use of this technology. The Cry1AbMod toxin was engineered to lack the alpha helix-1 of the parental Cry1Ab toxin and was shown to counter resistance to Cry1Ab and Cry1Ac toxins in different insect species including the fall armyworm Spodoptera frugiperda . In addition, Cry1AbMod showed enhanced toxicity to Cry1Ab-susceptible S. frugiperda populations. To gain insights into the mechanisms of this Cry1AbMod-enhanced toxicity, we isolated the Cry1AbMod toxin binding proteins from S. frugiperda brush border membrane vesicles (BBMV), which were identified by pull-down assay and liquid chromatography-tandem mass spectrometry (LC–MS/MS). The LC–MS/MS results indicated that Cry1AbMod toxin could bind to four classes of aminopeptidase (N1, N3, N4 y N5) and actin, with the highest amino acid sequence coverage acquired for APN 1 and APN4. In addition to these proteins, we found other proteins not previously described as Cry toxin binding proteins. This is the first report that suggests the interaction between Cry1AbMod and APN in S. frugiperda . |
| Databáze: | OpenAIRE |
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