MASS MORTALITY ASSOCIATED WITH KOI HERPESVIRUS IN WILD COMMON CARP IN CANADA
Autor: | S. Russell, Roselynn M. W. Stevenson, John S. Lumsden, Veronique LePage, Sandra Edes, Laura M. Hawley, L. Al-Hussinee, Kyle A. Garver, Elizabeth Wright, Tamara Schroeder, Brian W. Souter, Stephen Lord, Elena Contador |
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Rok vydání: | 2010 |
Předmět: |
Male
Canada Carps Interstitial nephritis Zoology Columnaris law.invention Fish Diseases Common carp law medicine Animals Koi herpesvirus Carp Herpesviridae Ecology Evolution Behavior and Systematics Polymerase chain reaction Ecology biology Reverse Transcriptase Polymerase Chain Reaction Aquatic animal Herpesviridae Infections Viral Load biology.organism_classification medicine.disease DNA Viral Female Viral load |
Zdroj: | Journal of Wildlife Diseases. 46:1242-1251 |
ISSN: | 0090-3558 |
DOI: | 10.7589/0090-3558-46.4.1242 |
Popis: | Koi herpesvirus (KHV) was identified as being associated with more than one mortality event affecting common carp in Canada. The first was an extensive mortality event that occurred in 2007 in the Kawartha Lakes region, Ontario, affecting Lakes Scugog and Pigeon. Fish had branchial necrosis and hepatic vasculitis with an equivocal interstitial nephritis. Several fish also had branchial columnaris. Subsequent mortality events occurred in 2008 in additional bodies of water in south-central Ontario, such as Lake Katchewanooka and outside of Ontario in Lake Manitoba, Manitoba. Koi herpesvirus was detected in fish submitted for examination from all of these lakes by polymerase chain reaction (PCR), and sequence of the PCR product revealed 100% homology to KHV strains U and I. Real-time PCR analysis of KHV-infected wild carp revealed viral loads ranging from 6.02×10(1) to 2.4×10(6) copies μg(-1) host DNA. This is the first report of KHV in Canada. |
Databáze: | OpenAIRE |
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