Demonstration of microchimerism in pregnant sows and effects of congenital PRRSV infection

Autor: Wander Van Breedam, Hans Nauwynck, Uladzimir Karniychuk, Claire Rogel-Gaillard, Nadine Van Roy
Přispěvatelé: Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University [Belgium] (UGENT), Center for Medical Genetics [Ghent], Ghent University Hospital, Génétique Animale et Biologie Intégrative (GABI), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Universiteit Gent = Ghent University [Belgium] (UGENT), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Karniychuk, Uladzimir U
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Male
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences
Swine
animal diseases
PATHOGENESIS
MATERNAL CELLS
Polymerase Chain Reaction
law.invention
Pathogenesis
0302 clinical medicine
law
Pregnancy
NONINVASIVE PRENATAL-DIAGNOSIS
Polymerase chain reaction
In Situ Hybridization
Fluorescence
0303 health sciences
lcsh:Veterinary medicine
030219 obstetrics & reproductive medicine
Microchimerism
POLYMERASE CHAIN-REACTION
3. Good health
In utero
Fish
Female
RESPIRATORY SYNDROME VIRUS
Porcine Reproductive and Respiratory Syndrome
Short Report
IN-UTERO
Biology
PERIPHERAL-BLOOD
Chimerism
03 medical and health sciences
FETUSES
medicine
Animals
Porcine respiratory and reproductive syndrome virus
Veterinary Sciences
030304 developmental biology
Fetus
General Veterinary
IDENTIFICATION
DNA
Porcine reproductive and respiratory syndrome virus
biology.organism_classification
medicine.disease
veterinary(all)
Immunology
lcsh:SF600-1100
UMBILICAL-CORD BLOOD
Zdroj: Veterinary Research
Veterinary Research, BioMed Central, 2012, 43, online (march), Non paginé. ⟨10.1186/1297-9716-43-19⟩
Veterinary Research march (43, online), Non paginé. (2012)
VETERINARY RESEARCH
Veterinary Research, Vol 43, Iss 1, p 19 (2012)
ISSN: 0928-4249
1297-9716
DOI: 10.1186/1297-9716-43-19⟩
Popis: The presence of foreign cells within the tissue/circulation of an individual is described as microchimerism. The main purpose of the present investigation was to study if microchimerism occurs in healthy sows/fetuses and if porcine reproductive and respiratory syndrome virus (PRRSV) infection influences this phenomenon. Six dams were inoculated intranasally with PRRSV and three non-inoculated dams served as controls. Male DNA was detected in female fetal sera of all dams via PCR. Male DNA was also detected in the maternal circulation. Sex-typing FISH showed the presence of male cells in the female fetal organs and vice versa. PRRSV infection did not influence microchimerism, but might misuse maternal and sibling microchimeric cells to enter fetuses.
Databáze: OpenAIRE
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