Delineation of a novel environmental phylogroup of the genus Acinetobacter encompassing Acinetobacter terrae sp. nov., Acinetobacter terrestris sp. nov. and three other tentative species
| Autor: | Lenka Radolfová-Křížová, Bruno S. Lopes, Petra Španělová, Renáta Šafránková, Alexandr Nemec, Martina Maixnerova, Ondrej Šedo, Matěj Nemec, Paul G. Higgins |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
DNA
Bacterial Turkey Zoology Fresh Water Biology Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences Germany RNA Ribosomal 16S Animals Clade Ecosystem Phylogeny Soil Microbiology Ecology Evolution Behavior and Systematics Czech Republic Netherlands 030304 developmental biology Base Composition 0303 health sciences Acinetobacter Phylogenetic tree 030306 microbiology Nucleic Acid Hybridization Sequence Analysis DNA biology.organism_classification Bacterial Typing Techniques Taxon Scotland Genus Acinetobacter Genes Bacterial |
| Zdroj: | Systematic and Applied Microbiology. 44:126217 |
| ISSN: | 0723-2020 |
| Popis: | This study aimed to define the taxonomic position and structure of a novel, taxonomically unique group of 26 Acinetobacter strains, provisionally designated Taxon 24 (T24). The strains were recovered from soil and freshwater ecosystems (n = 21) or animals (n = 5) in Czechia, Scotland, Germany, the Netherlands and Turkey between 1993 and 2015. They were non-glucose-acidifying, nonhemolytic, nonproteolytic, growing at 32 °C and on acetate and ethanol as single carbon sources, but not on 4-hydroxybenzoate and mostly not at 37 °C. Their whole-genome sequences were 3.0-3.7 Mb in size, with GC contents of 39.8-41.3%. Based on core genome phylogenetic analysis, the 26 strains formed a distinct clade within the genus Acinetobacter, with strongly supported subclades termed T24A (n = 11), T24B (n = 8), T24C (n = 2), T24D (n = 3) and T24E (n = 2). The internal genomic ANIb values for these subclades were94.8%, while the ANIb values between them were92.5%. The results of MALDI-TOF MS-based analyses agreed with this classification. The five subclades differed from each other in the results of one to six carbon source assimilation tests. Given the genomic and phenotypic distinctness, internal coherence, numbers of available strains and geographically diverse origin of T24A and T24B, we propose the names Acinetobacter terrae sp. nov. and Acinetobacter terrestris sp. nov. for these two taxa, respectively. The type strains are ANC 4282 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect