High-productivity lipid production using mixed trophic state cultivation of Auxenochlorella (Chlorella) protothecoides
| Autor: | Nicholas M. White, Hamid Rismani-Yazdi, Kristin H. Hampel, F. C. Thomas Allnutt, Christopher D. Lane, Kenneth M. Moats, Ben A. Kessler |
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| Rok vydání: | 2014 |
| Předmět: |
Manihot
Photochemistry Biomass Bioengineering Chlorella Auxenochlorella Raw material complex mixtures Bioreactors Oxazines Botany Food science Biodiesel biology Hydrolysis Fatty Acids food and beverages Starch General Medicine biology.organism_classification Lipids Aerobiosis Carbon Algae fuel Glucose Biofuel Biofuels Biodiesel production Fermentation Seeds Biotechnology |
| Zdroj: | Bioprocess and Biosystems Engineering. 38:639-650 |
| ISSN: | 1615-7605 1615-7591 |
| DOI: | 10.1007/s00449-014-1303-5 |
| Popis: | A mixed trophic state production process for algal lipids for use as feedstock for renewable biofuel production was developed and deployed at subpilot scale using a green microalga, Auxenochlorella (Chlorella) protothecoides. The process is composed of two separate stages: (1) the photoautotrophic stage, focused on biomass production in open ponds, and (2) the heterotrophic stage focused on lipid production and accumulation in aerobic bioreactors using fixed carbon substrates (e.g., sugar). The process achieved biomass and lipid productivities of 0.5 and 0.27 g/L/h that were, respectively, over 250 and 670 times higher than those obtained from the photoautotrophic cultivation stage. The biomass oil content (over 60 % w/DCW) following the two-stage process was predominantly monounsaturated fatty acids (~82 %) and largely free of contaminating pigments that is more suitable for biodiesel production than photosynthetically generated lipid. Similar process performances were obtained using cassava hydrolysate as an alternative feedstock to glucose. |
| Databáze: | OpenAIRE |
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