Effect of prostaglandin A1 in the induction of stress proteins in Aedes albopictus cells
| Autor: | M. A. Rebello, J.A Barbosa |
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| Rok vydání: | 1998 |
| Předmět: |
Aedes albopictus
Physiology Immunology Cell Biophysics Prostaglandin Biochemistry chemistry.chemical_compound Immune system Aedes medicine Protein biosynthesis Animals General Pharmacology Toxicology and Pharmaceutics lcsh:QH301-705.5 Polyacrylamide gel electrophoresis Heat-Shock Proteins lcsh:R5-920 Prostaglandins A biology Cell growth General Neuroscience Cell Biology General Medicine Aedes albopictus cells biology.organism_classification Molecular biology stress proteins Hsp70 medicine.anatomical_structure lcsh:Biology (General) chemistry prostaglandin lcsh:Medicine (General) Cell Division |
| Zdroj: | Brazilian Journal of Medical and Biological Research, Vol 31, Iss 4, Pp 499-503 (1998) Brazilian Journal of Medical and Biological Research, Vol 31, Iss 4, p 499 (1998) Brazilian Journal of Medical and Biological Research, Volume: 31, Issue: 4, Pages: 499-503, Published: APR 1998 |
| ISSN: | 0100-879X |
| Popis: | Prostaglandins are natural fatty acid derivatives with diverse physiological effects, including immune function and the control of cell growth. While the action of prostaglandins in the induction of stress proteins in vertebrate cells is well documented, their functions in invertebrate cells have been poorly investigated. The purpose of the present study was to investigate the effect of prostaglandin A1 (PGA1; 0.25, 1.25 and 12.5 micrograms/ml) on protein synthesis during the growth of Aedes albopictus cells. We found that PGA1 stimulates the synthesis of several polypeptides with molecular masses of 87, 80, 70, 57, 29, 27 and 23 kDa in Aedes albopictus cells. When the proteins induced by PGA1 and those induced by heat treatment were compared by polyacrylamide gel electrophoresis, PGA1 was found to induce the stress proteins. The HSP70 family and the low-molecular weight polypeptides (29 and 27 kDa, respectively) were induced by PGA1 in the lag phase. We also observed that PGA1 is able to induce a 23-kDa polypeptide independently of the growth phase of the cell. |
| Databáze: | OpenAIRE |
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