Vascular endothelial growth factor induces mRNA expression of pro-inflammatory factors in the uterine cervix of mice
| Autor: | Ellie McCabe, Jennifer L. Cecile, Chishimba Nathan Mowa, Veronica Minkiewicz, Bao-Tran Nguyen |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Lipopolysaccharides
Vascular Endothelial Growth Factor A medicine.medical_specialty Angiogenesis Vascular permeability Inflammation Cervix Uteri General Biochemistry Genetics and Molecular Biology Mice chemistry.chemical_compound Pregnancy Internal medicine Animals Medicine RNA Messenger Interleukin-6 Tumor Necrosis Factor-alpha business.industry General Medicine Vascular endothelial growth factor Vascular endothelial growth factor B Vascular endothelial growth factor A Endocrinology Gene Expression Regulation Vascular endothelial growth factor C chemistry Female Tumor necrosis factor alpha medicine.symptom business |
| Zdroj: | Biomedical Research. 33:363-372 |
| ISSN: | 1880-313X 0388-6107 |
| DOI: | 10.2220/biomedres.33.363 |
| Popis: | Inflammation is believed to play a role in uterine cervical remodeling and infection-induced preterm labor. One of the distinct features of remodeling uterine cervix is presence of prominent vascular events, such as angiogenesis, vasodilation, and vascular permeability. Although the functional significance of these features is not yet clear, we know that in most tissue types, vascular remodeling is intricately intertwined with inflammation. Since vascular endothelial growth factor (VEGF) is the major architect of vascular remodeling, we sought to examine and elucidate the potential relationship between VEGF and inflammation in the uterine cervix of non-pregnant mice. The animals used were divided into 4 treatment groups: A) negative control (vehicle only), B) positive control (lipopolysaccharide, LPS), C) recombinant VEGF-164 protein, and D) LPS + VEGF blocker (n = 3). After the appropriate treatments, the uterine cervices were harvested and analyzed using real-time PCR and confocal fluorescence microscopy. Results showed that exogenous VEGF upregulates expression of interleukin (IL)-6 and tumor necrosis factor (TNF)-α mRNAs, whereas VEGF blocker partially diminishes the LPS-induced expression of pro-inflammatory factors compared to the positive control group. We conclude that a positive feed-forward relationship likely exists between VEGF and inflammation in the uterine cervix, thus implicating VEGF in inflammation-induced preterm labor. |
| Databáze: | OpenAIRE |
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