Acute In Vivo Electrophysiological Recordings of Local Field Potentials and Multi-unit Activity from the Hyperdirect Pathway in Anesthetized Rats
| Autor: | Christoph van Riesen, Christopher Güttler, Dieu-Huong Nguyen, Jens K. Haumesser, Andrea A. Kühn, Maximilian H. Beck, Johanna Kühn |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.diagnostic_test General Immunology and Microbiology Computer science General Chemical Engineering General Neuroscience Local field potential General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Electrophysiology 030104 developmental biology 0302 clinical medicine Calcium imaging In vivo medicine Biological neural network Premovement neuronal activity Functional magnetic resonance imaging Electrocorticography Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Journal of Visualized Experiments. |
| ISSN: | 1940-087X |
| DOI: | 10.3791/55940-v |
| Popis: | Converging evidence shows that many neuropsychiatric diseases should be understood as disorders of large-scale neuronal networks. To better understand the pathophysiological basis of these diseases, it is necessary to precisely characterize in which way the processing of information is disturbed between the different neuronal parts of the circuit. Using extracellular in vivo electrophysiological recordings, it is possible to accurately delineate neuronal activity within a neuronal network. The application of this method has several advantages over alternative techniques, e.g., functional magnetic resonance imaging and calcium imaging, as it allows a unique temporal and spatial resolution and does not rely on genetically engineered organisms. However, the use of extracellular in vivo recordings is limited since it is an invasive technique that cannot be universally applied. In this article, a simple and easy to use method is presented with which it is possible to simultaneously record extracellular potentials such as local field potentials and multiunit activity at multiple sites of a network. It is detailed how a precise targeting of subcortical nuclei can be achieved using a combination of stereotactic surgery and online analysis of multi-unit recordings. Thus, it is demonstrated, how a complete network such as the hyperdirect cortico-basal ganglia loop can be studied in anesthetized animals in vivo. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|