Evaluation of a Group A Streptococcus synthetic oligosaccharide as vaccine candidate
| Autor: | William J. Christ, Marilena Gallotta, Giuliano Bensi, Sabrina Capo, Guido Grandi, John Pena, Paola Fontani, Erwin Swennen, Peter H. Seeberger, Roberto Adamo, Daniela Proietti, Giada Buffi, Emiliano Chiarot, Anna Kabanova, Vittoria Pinto, Daniele Casini, Rino Rappuoli, Francesco Berti, Emilia Cappelletti, David A. G. Skibinski, Paolo Costantino, Maria Rosaria Romano, Immaculada Margarit, A. Stewart Campbell |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Serum
Glycoconjugate Streptococcus pyogenes Oligosaccharides Microbiology Mice Antigen Phagocytosis Animals chemistry.chemical_classification Antiserum Vaccines Synthetic Vaccines Conjugate General Veterinary General Immunology and Microbiology biology Immunogenicity Streptococcal Vaccines Public Health Environmental and Occupational Health Oligosaccharide Vaccination Infectious Diseases chemistry biology.protein Molecular Medicine Female Bacterial antigen Rabbits Antibody |
| Zdroj: | Vaccine. 29(1) |
| ISSN: | 1873-2518 |
| Popis: | Bacterial infections caused by Group A Streptococcus (GAS) are a serious health care concern that currently cannot be prevented by vaccination. The GAS cell-wall polysaccharide (GAS-PS) is an attractive vaccine candidate due to its constant expression pattern on different bacterial strains and protective properties of anti-GAS-PS antibodies. Here we report for the first time the immunoprotective efficacy of glycoconjugates with synthetic GAS oligosaccharides as compared to those containing the native GAS-PS. A series of hexa- and dodecasaccharides based on the GAS-PS structure were prepared by chemical synthesis and conjugated to CRM(197). When tested in mice, the conjugates containing the synthetic oligosaccharides conferred levels of immunoprotection comparable to those elicited by the native conjugate. Antisera from immunized rabbits promoted phagocytosis of encapsulated GAS strains. Furthermore we discuss variables that might correlate with glycoconjugate immunogenicity and demonstrate the potential of the synthetic approach that benefits from increased antigen purity and facilitated manufacturing. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect