Acute oral toxicity of 3-MCPD mono- and di-palmitic esters in Swiss mice and their cytotoxicity in NRK-52E rat kidney cells
| Autor: | Xuebing Xu, Wei Luo, Liangli Lucy Yu, Haiming Shi, Fang Qin, Man Liu, Boyan Gao, Ping-Ping Wu, Ai-Niu Ma, Yuan-Rong Jiang |
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| Rok vydání: | 2012 |
| Předmět: |
Glycerol
medicine.medical_specialty Time Factors Palmitic Acid Administration Oral alpha-Chlorohydrin Rat kidney Toxicology Median lethal dose Serum urea Cell Line Mice chemistry.chemical_compound Internal medicine 3-MCPD medicine Animals Urea Oral toxicity Cytotoxicity Creatinine Dose-Response Relationship Drug Chemistry Body Weight Organ Size General Medicine Rats Endocrinology Liver Biochemistry Toxicity Food Science |
| Zdroj: | Food and Chemical Toxicology. 50:3785-3791 |
| ISSN: | 0278-6915 |
| DOI: | 10.1016/j.fct.2012.07.038 |
| Popis: | The acute oral toxicity of 1-palmitoyl-3-chloropropanediol (3-MCPD 1-monopalmitate) and 1,2-bis-palmitoyl-3-chloropropanediol (3-MCPD dipalmitate) in Swiss mice were examined, along with their cytotoxicity in NRK-52E rat kidney cells. LD50 (median lethal dose) value of 3-MCPD 1-monopalmitate was determined 2676.81 mg/kg body weight (BW). The results showed that 3-MCPD 1-monopalmitate dose-dependently decreased the mean body weight, and caused significant increase of serum urea nitrogen and creatinine in dead mice compared to the control and survived mice. Major histopathological changes in mice fed 3-MCPD 1-monopalmitate were renal tubular necrosis, protein casts and spermatids decrease in the seminiferous tubules. According to the limit test for 3-MCPD dipalmitate, LD50 value of 3-MCPD dipalmitate was presumed to be greater than 5000 mg/kg BW. Obvious changes were not observed on mean body weight, absolute and relative organ weight or serum urea nitrogen and creatinine levels in mice fed 3-MCPD dipalmitate. However, renal tubular necrosis, protein casts and spermatids decrease were also observed in the dead mice. In addition, MTT and LDH assay results only showed the cytotoxicity of 3-MCPD 1-monopalmitate in NRK-52E rat kidney cells in a dose-dependent manner. Together, the results indicated a greater toxicity of 3-MCPD 1-monopalmitate compared to 3-MCPD dipalmitate. |
| Databáze: | OpenAIRE |
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