Discrete Nanoscale Distribution of Hair Lipids Fails to Provide Humidity Resistance
Autor: | Yann Waye Keuong, Christian Mazilier, Nawel Baghdadli, Ariane Deniset-Besseau, Isabelle Pasini, Alexandre Dazzi, Lucien Bildstein |
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Rok vydání: | 2020 |
Předmět: |
Spectrophotometry
Infrared Infrared Infrared spectroscopy Microscopy Atomic Force 010402 general chemistry Methylation 01 natural sciences Analytical Chemistry Microscopy Stratum corneum medicine Humans Molecule Nanoscopic scale Esterification integumentary system Chemistry Atomic force microscopy 010401 analytical chemistry Water Humidity Lipids Nanostructures 0104 chemical sciences medicine.anatomical_structure Biophysics Hair |
Zdroj: | Analytical Chemistry. 92:11498-11504 |
ISSN: | 1520-6882 0003-2700 |
Popis: | The subcellular distribution of lipids in human hair was investigated to better understand their role in water permeability. Unlike stratum corneum where lipids are organized under a precisely ordered continuous structure, the removal of free lipids in hair does not lead to an increase of water permeability. Esterified and CH2-enriched molecules were tracked at a 10 nm resolution by infrared nanospectroscopy (atomic force microscopy coupled to infrared spectroscopy, AFMIR). Free and bound lipids in the 25 nm thick intercellular spaces were directly detected for the first time, further substantiating the potential of AFMIR to study complex biomaterials. We observed that they were mostly found accumulated in some regions of the external cuticle layers, as "hotspots" in nonkeratinous portions of the internal cortex, and that they do not seem to modulate much the water exchanges due to their discrete distribution throughout the hair fiber. |
Databáze: | OpenAIRE |
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