N-methyl-N-nitrosourea-induced neuronal cell death in a large animal model of retinal degeneration in vitro
| Autor: | Linnéa Taylor, Fredrik Ghosh, Karin Arnér |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Retinal degeneration
Programmed cell death Pathology medicine.medical_specialty Retinal Bipolar Cells genetic structures Swine Ependymoglial Cells Biology Calbindin Retina 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound 0302 clinical medicine medicine Animals Cell Death Retinal Degeneration Retinal Methylnitrosourea medicine.disease Immunohistochemistry Sensory Systems Cell biology Transplantation Ophthalmology Disease Models Animal medicine.anatomical_structure nervous system Gliosis chemistry 030221 ophthalmology & optometry Neuroglia sense organs medicine.symptom 030217 neurology & neurosurgery Photoreceptor Cells Vertebrate Retinal Neurons |
| Zdroj: | Experimental eye research. 148 |
| ISSN: | 1096-0007 |
| Popis: | N-methyl-N-nitrosourea (MNU) has been reported to induce photoreceptor-specific degeneration with minimal inner retinal impact in small animals in vivo. Pending its use within a retinal transplantation paradigm, we here explore the effects of MNU on outer and inner retinal neurons and glia in an in vitro large animal model of retinal degeneration. The previously described degenerative culture explant model of adult porcine retina was used and compared with explants receiving 10 or 100 μg/ml MNU (MNU10 and MNU100) supplementation. All explants were kept for 5 days in vitro, and examined for morphology as well as for glial and neuronal immunohistochemical markers. Rhodopsin-labeled photoreceptors were present in all explants. The number of cone photoreceptors (transducin), rod bipolar cells (PKC) and horizontal cells (calbindin) was significantly lower in MNU treated explants (p |
| Databáze: | OpenAIRE |
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