Structural and Oxidative Changes in the Kidney of Crucian Carp Induced by Silicon-Based Quantum Dots
| Autor: | Radu Burlacu, Otilia Zarnescu, Marieta Costache, Andreea Iren Serban, Andreia Cristina Staicu, Maria Cristina Munteanu, Cornelia Sima, Loredana Stanca, Anca Dinischiotu, S.N. Petrache |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Silicon
medicine.medical_specialty Carps Glutathione reductase quantum dots Kidney medicine.disease_cause Article Catalysis lcsh:Chemistry Inorganic Chemistry Superoxide dismutase Lipid peroxidation chemistry.chemical_compound Internal medicine medicine Animals Physical and Theoretical Chemistry lcsh:QH301-705.5 Molecular Biology Spectroscopy Glutathione Transferase chemistry.chemical_classification Glutathione Peroxidase biology Superoxide Dismutase Glutathione peroxidase Organic Chemistry General Medicine Glutathione Catalase Computer Science Applications Oxidative Stress Glutathione Reductase medicine.anatomical_structure Endocrinology lcsh:Biology (General) lcsh:QD1-999 chemistry biology.protein Lipid Peroxidation fish kidney Oxidation-Reduction Oxidative stress |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 13, Iss 8, Pp 10193-10211 (2012) Volume 13 Issue 8 Pages 10193-10211 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms130810193 |
| Popis: | Silicon-based quantum dots were intraperitoneally injected in Carassius auratus gibelio specimens and, over one week, the effects on renal tissue were investigated by following their distribution and histological effects, as well as antioxidative system modifications. After three and seven days, detached epithelial cells from the basal lamina, dilated tubules and debris in the lumen of tubules were observed. At day 7, nephrogenesis was noticed. The reduced glutathione (GSH) concentration decreased in the first three days and started to rise later on. The superoxide dismutase (SOD) activity increased only after one week, whereas catalase (CAT) was up-regulated in a time-dependent manner. The activities of glutathione reductase (GR) and glutathione peroxidise (GPX) decreased dramatically by approximately 50% compared to control, whereas the glutathione-S-transferase (GST) and glucose-6-phosphate dehydrogenase (G6PDH) increased significantly after 3 and 7 days of treatment. Oxidative modifications of proteins and the time-dependent increase of Hsp70 expression were also registered. Our data suggest that silicon-based quantum dots induced oxidative stress followed by structural damages. However, renal tissue is capable of restoring its integrity by nephron development. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|