High concentrations of α-defensins in plasma and bronchoalveolar lavage fluid of patients with acute respiratory distress syndrome
| Autor: | Masamitsu Nakazato, Masatoshi Tokojima, Arisa Sano, Jun-ichi Ashitani, Hiroshi Mukae, Yasuji Arimura |
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| Rok vydání: | 2004 |
| Předmět: |
Male
alpha-Defensins ARDS Neutrophils Alpha (ethology) Enzyme-Linked Immunosorbent Assay Lung injury General Biochemistry Genetics and Molecular Biology Pathogenesis Bone Marrow Granulocyte Colony-Stimulating Factor medicine Humans Interleukin 8 General Pharmacology Toxicology and Pharmaceutics Chromatography High Pressure Liquid Aged Respiratory Distress Syndrome integumentary system medicine.diagnostic_test business.industry Interleukin-8 fungi Interleukin hemic and immune systems Lung Injury General Medicine Middle Aged respiratory system bacterial infections and mycoses medicine.disease Kinetics Bronchoalveolar lavage medicine.anatomical_structure Immunology Female Bone marrow business Bronchoalveolar Lavage Fluid |
| Zdroj: | Life Sciences. 75:1123-1134 |
| ISSN: | 0024-3205 |
| Popis: | alpha-Defensins, antimicrobial peptides localized in neutrophils, participate in tissue damage through their cytotoxic effects in neutrophil-mediated pulmonary diseases. Neutrophils play an important role in the pathogenesis of acute respiratory distress syndrome (ARDS). We measured alpha-defensins levels in plasma and bronchoalveolar lavage fluid (BALF) of ARDS patients to assess the kinetics of alpha-defensins in ARDS. Plasma alpha-defensins levels were higher in ARDS patients than in control subjects, and BALF levels were also higher in ARDS patients than in control subjects. In ARDS, BALF alpha-defensins levels correlated with those of interleukin (IL)-8, and plasma alpha-defensins levels also correlated with Lung Injury Score. Peripheral neutrophil alpha-defensins contents were higher in ARDS than the control. IL-8 dose-dependently stimulated alpha-defensins release from cultured neutrophils and these levels were higher in ARDS than the control. Reverse-phase high performance liquid chromatography showed high plasma levels of pro-defensins, precursors of alpha-defensins from the bone marrow in ARDS, although alpha-defensins in peripheral and BALF neutrophils were mature type. In conclusion, high plasma alpha-defensins in ARDS patients result from the release of pro-defensins from bone marrow, rather than mature alpha-defensins from neutrophils that accumulate in the alveolar space. The alpha-defensins contents of peripheral neutrophils in ARDS are higher and easier to release than control. |
| Databáze: | OpenAIRE |
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