Repeatability of the ACTH stimulation test as reflected by salivary cortisol response in healthy horses
| Autor: | Rupert M. Bruckmaier, J.H. van der Kolk, G. Schüpbach-Regula, M.D. Scheidegger, Alessandra Ramseyer, V. Gerber |
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| Rok vydání: | 2015 |
| Předmět: |
Male
endocrine system Saliva medicine.medical_specialty Hydrocortisone 040301 veterinary sciences Adrenocorticotropic hormone 0403 veterinary science Endocrinology Food Animals Adrenocorticotropic Hormone Internal medicine medicine Animals Horses medicine.diagnostic_test Adrenal cortex business.industry 0402 animal and dairy science ACTH stimulation test Horse 04 agricultural and veterinary sciences Repeatability 040201 dairy & animal science medicine.anatomical_structure Warmblood Animal Science and Zoology Female business hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Domestic animal endocrinology. 57 |
| ISSN: | 1879-0054 |
| Popis: | The aim of this study was to further characterize the ACTH stimulation test as reflected by salivary cortisol response and to measure the short- and long-term repeatability of it in healthy horses as a tool to assess the capacity of the adrenal cortex to secrete cortisol. Nineteen healthy horses were subjected to 3 ACTH stimulation tests. Intervals were 2 wk and 5 mo between the first and second and the second and third tests, respectively. A dose of 1-μg/kg BW synthetic ACTH was injected intravenously. Saliva samples were collected at baseline and at 30, 60, 90, 120, 150, and 180 min after administration for cortisol measurements using a competitive enzyme immunoassay. A repeated measures ANOVA was used to compare values within and among horses. Mean ± SD total increase in cortisol concentrations integrated over the entire sampling period was 34.5 ± 11.0 ng/mL. The highest measured concentration at a single time point was 9.7 ± 2.7 ng/mL and was reached after 122 ± 22 min. For the short- and long-term repeatability, intraclass correlation coefficient was 0.90 and 0.33, respectively. The 3 ACTH stimulation tests results differed significantly among (P < 0.00001) but not within (P = 0.538) individual horses. The Freiberger stallions had a higher salivary cortisol baseline concentration and a lower response to ACTH stimulation as compared with Warmblood mares and geldings. The present study confirmed that the administration of ACTH in healthy horses reliably stimulates the salivary secretion of cortisol and shows that the test is repeatable in the short- and long-term. |
| Databáze: | OpenAIRE |
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