Localization of the 5-hydroxytryptamine 4 receptor in equine enteric neurons and extrinsic sensory fibers
| Autor: | Fiorella Giancola, Noemi Romagnoli, Roberto Chiocchetti, R. De Giorgio, Paolo Clavenzani, Francesca Bianco, Cristiano Bombardi, S. Iusco, Anna Maria Rambaldi, Claudio Tagliavia |
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| Přispěvatelé: | Giancola, Fiorella, Rambaldi, ANNA MARIA, Bianco, Francesca, Iusco, Simona, Romagnoli, Noemi, Tagliavia, Claudio, Bombardi, Cristiano, Clavenzani, Paolo, DE GIORGIO, Roberto, Chiocchetti, Roberto |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Pathology Serotonin Sensory Receptor Cells Physiology Prokinetic Myenteric Plexus Ileum Sensory system Biology Endocrine and Autonomic System Descending colon 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Animals Horses Receptor Endocrine and Autonomic Systems Gastroenterology musculoskeletal system Ganglion Gastrointestinal Tract Lethal white foal syndrome 030104 developmental biology medicine.anatomical_structure Endocrinology Duodenum 030211 gastroenterology & hepatology Enteric nervous system Receptors Serotonin 5-HT4 5-hydroxytryptamine receptor type 4 |
| Popis: | Background Serotonin plays a pivotal role in regulating gut motility, visceral sensitivity, and fluid secretion via specific receptors. Among these receptors, 5-HT4 exerts a prominent control on gut motor function. Although the prokinetic effect exerted by 5-HT4 agonists is well known, the cellular sites of 5-HT4 expression remain poorly understood in large mammals, e.g., horses. In this study, we evaluated the distribution of 5-HT4 in the horse intestine and in foals with enteric aganglionosis, reminiscent of human Hirschsprung's disease. Methods The intestine and spinal ganglia were obtained from three healthy horses and two foals with hereditary ileocolonic aganglionosis. Tissues were processed for immunohistochemistry using a specific antibody to 5-HT4 and a variety of neuronal markers. Myenteric and submucosal plexus 5-HT4-immunoreactive (IR) neurons were quantified as relative percentage (mean±SD) to the total number of neurons counted. Furthermore, the density of 5-HT4-IR nerve fibers was evaluated in the mucosa and tunica muscularis. Key Results The 5-HT4 immunoreactivity was localized to large percentages of myenteric neurons ranging from 28±9% (descending colon) to 63±19% (ileum), and submucosal neurons ranging from 54±6% (ileum) to 68±14% (duodenum). The 5-HT4-immunoreactivity was co-expressed by some substance P-IR (SP-IR) spinal ganglion neurons and extrinsic sensory fibers of aganglionic foals. Conclusions & Inferences The presence of 5-HT4 in many enteric and extrinsic sensory neurons and nerve fibers provides solid morphological evidence of the cellular sites of 5-HT4 expression in horses. The evidence of SP-IR sensory neurons positive for 5-HT4 suggests its role in visceral sensitivity. |
| Databáze: | OpenAIRE |
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