Characterization of the Burkholderia pseudomallei K96243 Capsular Polysaccharide I Coding Region
| Autor: | Richard W. Titball, Sarah V. Harding, Jon Cuccui, Joann L. Prior, Timothy S. Milne, Mitali Sarkar-Tyson, Andrew E. Scott, Brendan W. Wren, Nicholas J. Harmer, Alison J. George, Rachel E. Dean |
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| Rok vydání: | 2012 |
| Předmět: |
Burkholderia pseudomallei
Melioidosis Virulence Factors Blotting Western Immunology Mutant Fluorescent Antibody Technique Virulence Locus (genetics) Vaccines Attenuated Microbiology Bacterial genetics Gene Knockout Techniques Mice medicine Animals Gene Mice Inbred BALB C biology Polysaccharides Bacterial biochemical phenomena metabolism and nutrition bacterial infections and mycoses medicine.disease biology.organism_classification Phenotype Virology Biosynthetic Pathways Infectious Diseases Genetic Loci Multigene Family Microbial Immunity and Vaccines Bacterial Vaccines bacteria Female Parasitology |
| Zdroj: | Infection and Immunity. 80:1209-1221 |
| ISSN: | 1098-5522 0019-9567 |
| Popis: | Burkholderia pseudomallei is the causative agent of melioidosis, a disease endemic to regions of Southeast Asia and Northern Australia. Both humans and a range of other animal species are susceptible to melioidosis, and the production of a group 3 polysaccharide capsule in B. pseudomallei is essential for virulence. B. pseudomallei capsular polysaccharide (CPS) I comprises unbranched manno -heptopyranose residues and is encoded by a 34.5-kb locus on chromosome 1. Despite the importance of this locus, the role of all of the genes within this region is unclear. We inactivated 18 of these genes and analyzed their phenotype using Western blotting and immunofluorescence staining. Furthermore, by combining this approach with bioinformatic analysis, we were able to develop a model for CPS I biosynthesis and export. We report that inactivating gmhA , wcbJ , and wcbN in B. pseudomallei K96243 retains the immunogenic integrity of the polysaccharide despite causing attenuation in the BALB/c murine infection model. Mice immunized with the B. pseudomallei K96243 mutants lacking a functional copy of either gmhA or wcbJ were afforded significant levels of protection against a wild-type B. pseudomallei K96243 challenge. |
| Databáze: | OpenAIRE |
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