Identification of calconectin, a calcium-binding protein specifically expressed by the mantle ofPinctada margaritifera

Autor: Denis Duplat, M. Puisségur, Hélène Boulzaguet, Daniel Y. Sellos, Evelyne Lopez, Laurent Bédouet, Marthe Rousseau, Christian Milet, A. Van Wormhoudt
Přispěvatelé: Biologie des organismes marins et écosystèmes (BOME), Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Muséum national d'Histoire naturelle (MNHN), Muséum national d'Histoire naturelle (MNHN)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2006
Předmět:
Biomineralization
DNA
Complementary
Molecular Sequence Data
Biophysics
Gene Expression
Mother-of-pearl
cDNA library
EF-hand
010402 general chemistry
01 natural sciences
Biochemistry
Pinctada margaritifera
03 medical and health sciences
Rapid amplification of cDNA ends
Structural Biology
Calcium-binding protein
Complementary DNA
Gene expression
[SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO]
Genetics
Animals
Amino Acid Sequence
Pinctada
Molecular Biology
Peptide sequence
030304 developmental biology
Calconectin
0303 health sciences
Base Sequence
Sequence Homology
Amino Acid
biology
Reverse Transcriptase Polymerase Chain Reaction
Calcium-Binding Proteins
F-hand
Cell Biology
biology.organism_classification
Molecular biology
0104 chemical sciences
Suppression subtractive hybridization
Zdroj: FEBS Letters
FEBS Letters, Wiley, 2006, 580, pp.2435-2441
FEBS Letters, 2006, 580, pp.2435-2441
ISSN: 0014-5793
1873-3468
Popis: Nacre or mother-of-pearl in the shell of Pinctada margaritifera is composed of 95–99% calcium carbonate and 1–5% organic matrix. In this study, we developed an original technique to characterize the genes differentially expressed in nacre-forming cells (NFC) by combining suppression subtractive hybridization (SSH), to establish a cDNA subtractive library, with rapid amplification of cDNA ends (RACE)-PCR. Seventy-two specific cDNA sequences have been obtained so far. These include a protein containing two EF-hand Ca2+-binding domains which was completely sequenced after amplification by RACE-PCR. Its specific expression as well as the specificity of the SSH method was confirmed by semi-quantitative RT-PCR on NFC and mantle cells.
Databáze: OpenAIRE
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