Classifying glycerol dehydratase by its functional residues and purifying selection in its evolution

Autor:	Andrés Escobar, Dolly Montoya Castaño
Rok vydání:	2010
Předmět:	type I functional divergence Phylogenetic tree molecular evolution Mutagenesis Glycerol dehydratase General Medicine Hypothesis Biology Bioinformatics Negative selection chemistry.chemical_compound hot pots chemistry Biochemistry glycerol dehydratase Molecular evolution I divergence Glycerol Clade
Zdroj:	Bioinformation
ISSN:	0973-2063 0973-8894
DOI:	10.6026/97320630005173
Popis:	Glycerol dehydratase (GD) catalyses glycerol reductive conversion to 3-hydroxypropanaldehyde (3-HPA), this being the first step required for the microbial conversion of glycerol to 1, 3 -propanodiol. GD has been functionally characterised to date and two main groups have been determined, one of them being vitamin B(12)-dependent and the other B(12)-independent. GD evolutionary history has been described and an exhaustive analysis made for detecting the functional residues responsible for type I divergence. GD phylogenetic tree topology was seen to be statistically robust and the data indicated strong purifying selection operating on the GD proteins within it. Two clades were indentified, one for vitamin B(12)-dependent and the other for B(12)- independent classes. The ancient hot-pot residues responsible for protein divergency for each clade were also identified. The basic evolutionary biology for GD proteins has been described, thereby opening the way forward for developing rational mutagenesis studies.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ec0f0dbecd52959f7893164795326f7 https://doi.org/10.6026/97320630005173 Zobrazit plný text záznamu