Role of miRNA-210, miRNA-21 and miRNA-126 as diagnostic biomarkers in colorectal carcinoma: impact of HIF-1α-VEGF signaling pathway
| Autor: | Elham Ahmed Hassan, Heba E M El-Deek, Eman Amer, Moataz Maher, Hala M El-Bader, Dina Sabry, Wael Fathy, Sahar E M El-Deek, Mona A.H. El-Baz |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Adenoma Male Vascular Endothelial Growth Factor A Colorectal cancer Clinical Biochemistry Adenocarcinoma medicine.disease_cause Metastasis 03 medical and health sciences 0302 clinical medicine microRNA VEGF Signaling Pathway medicine Biomarkers Tumor Humans Molecular Biology Survival rate Aged business.industry Cancer Cell Biology General Medicine Middle Aged medicine.disease Hypoxia-Inducible Factor 1 alpha Subunit Prognosis Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology Early Diagnosis 030220 oncology & carcinogenesis Cancer research Female business Carcinogenesis Colorectal Neoplasms Signal Transduction |
| Zdroj: | Molecular and cellular biochemistry. 454(1-2) |
| ISSN: | 1573-4919 |
| Popis: | Colorectal cancer (CRC) is a major cause of death worldwide. Novel non-invasive, high diagnostic value screening test is urgently needed to improve survival rate, treatment and prognosis. Stable, small, circulating microRNA (miRNA) offers unique opportunities for the early diagnosis of several diseases. It acts as tumor oncogenes or suppressors and involve in cell death, survival, and metastasis. Communication between miRNA and carcinogenesis is critical but it still not clear and needs further investigation. The aim of our study is to evaluate the role of miR-210, miR-21, miR-126, as non-invasive diagnostic biomarkers for screening, early detection of CRC, studying their correlation with prognostic variables, and clarifying the roles of miRNAs on HIF-1α-VEGF signaling pathway. The expression of miR-210, miR-21 and miR-126 was performed using qRT-PCR in adenocarcinoma (no = 35), adenomas (no = 51), and neoplasm free controls (no = 101). Serum levels of VEGF and HIF-1α was determined by ELISA Kit. The results show that the expression of miR-210, miR-21, VEGF, HIF-1α was significantly up-regulated while that miRNA-126 was down-regulated in both adenocarcinoma and adenomas compared with controls (p |
| Databáze: | OpenAIRE |
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