Quantitative evaluation of radiation-induced changes in sperm morphology and chromatin distribution
| Autor: | Karsten Rodenacker, Uta Jütting, Peter Gais, Georg Burger, Michaela Aubele, U. Hacker-Klom |
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| Rok vydání: | 1990 |
| Předmět: |
Male
endocrine system Biophysics Biology Radiation Dosage Pathology and Forensic Medicine Flow cytometry Mice chemistry.chemical_compound Endocrinology medicine Animals reproductive and urinary physiology Gametogenesis Genetics medicine.diagnostic_test urogenital system Cell Biology Hematology Spermatozoa Sperm Radiation effect Chromatin Cell biology chemistry Microscopy Electron Scanning Cytometry Spermatogenesis DNA |
| Zdroj: | Cytometry. 11:586-594 |
| ISSN: | 1097-0320 0196-4763 |
| DOI: | 10.1002/cyto.990110505 |
| Popis: | Sperm head cytometry provides a useful assay for the detection of radiation-induced damage in mouse germ cells. Exposure of the gonads to radiation is known to lead to an increase of diploid and higher polyploid sperm and of sperm with head shape abnormalities. In the pilot studies reported here quantitative analysis of the total DNA content, the morphology, and the chromatin distribution of mouse sperm was performed. The goal was to evaluate the discriminative power of features derived by high resolution image cytometry in distinguishing sperm of control and irradiated mice. Our results suggest that besides the induction of the above mentioned variations in DNA content and shape of sperm head, changes of the nonhomogeneous chromatin distribution within the sperm may also be used to quantify the radiation effect on sperm cells. Whereas the chromatin distribution features show larger variations for sperm 21 days after exposure (dpr), the shape parameters seem to be more important to discriminate sperm 35 dpr. This may be explained by differentiation processes, which take place in different stages during mouse spermatogenesis. |
| Databáze: | OpenAIRE |
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