Introducing Mammalian Cell Culture and Cell Viability Techniques in the Undergraduate Biology Laboratory
| Autor: | Travis Russell, Verónica A. Segarra, Michael G. Elias, Kristen Bowey-Dellinger, Dinene Crater, V. McNeil Coffield, Cynthia C. Vigueira, Todd A. Lyda, Michael H. Grider, Yewseok K. Suh, Kelli Sapp, Luke Dixon, Kristin M. Ackerman |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
QH301-705.5
education Cellular level Biology computer.software_genre General Biochemistry Genetics and Molecular Biology inquiry-based laboratory exercise Education 03 medical and health sciences 0302 clinical medicine mammalian cells Viability assay Biology (General) lcsh:QH301-705.5 cell viability lcsh:LC8-6691 lcsh:Special aspects of education General Immunology and Microbiology Multimedia LC8-6691 Mechanism (biology) 05 social sciences mammalian cell culture 050301 education Special aspects of education Biological safety lcsh:Biology (General) Cell culture 030220 oncology & carcinogenesis Curriculum General Agricultural and Biological Sciences 0503 education computer HeLa Cells |
| Zdroj: | Journal of Microbiology & Biology Education, Vol 18, Iss 2 (2017) Journal of Microbiology & Biology Education |
| ISSN: | 1935-7877 1935-7885 |
| DOI: | 10.1128/jmbe.v18i2.1264 |
| Popis: | Undergraduate students learn about mammalian cell culture applications in introductory biology courses. However, laboratory modules are rarely designed to provide hands-on experience with mammalian cells or teach cell culture techniques, such as trypsinization and cell counting. Students are more likely to learn about cell culture using bacteria or yeast, as they are typically easier to grow, culture, and manipulate given the equipment, tools, and environment of most undergraduate biology laboratories. In contrast, the utilization of mammalian cells requires a dedicated biological safety cabinet and rigorous antiseptic techniques. For this reason, we have devised a laboratory module and method herein that familiarizes students with common cell culture procedures, without the use of a sterile hood or large cell culture facility. Students design and perform a time-efficient inquiry-based cell viability experiment using HeLa cells and tools that are readily available in an undergraduate biology laboratory. Students will become familiar with common techniques such as trypsinizing cells, cell counting with a hemocytometer, performing serial dilutions, and determining cell viability using trypan blue dye. Additionally, students will work with graphing software to analyze their data and think critically about the mechanism of death on a cellular level. Two different adaptations of this inquiry-based lab are presented—one for non-biology majors and one for biology majors. Overall, these laboratories aim to expose students to mammalian cell culture and basic techniques and help them to conceptualize their application in scientific research. |
| Databáze: | OpenAIRE |
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