Urinary NID2 and TWIST1 methylation to augment conventional urine cytology for the detection of bladder cancer
| Autor: | Wen-Chi Foo, Richmond Owusu, Ajay Gopalakrishna, Michael R. Abern, Joseph J. Fantony, Brant A. Inman, Thomas A. Longo, Raymond S. Lance |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Cancer Research medicine.medical_specialty Urinary system Urology Urine 03 medical and health sciences 0302 clinical medicine Cytology Biomarkers Tumor Genetics medicine Humans Aged Urine cytology Carcinoma Transitional Cell Bladder cancer medicine.diagnostic_test business.industry Calcium-Binding Proteins Twist-Related Protein 1 Nuclear Proteins Cystoscopy General Medicine Methylation DNA Methylation Middle Aged medicine.disease 030104 developmental biology Urinary Bladder Neoplasms Oncology 030220 oncology & carcinogenesis DNA methylation Female business Cell Adhesion Molecules |
| Zdroj: | Cancer Biomarkers. 18:381-387 |
| ISSN: | 1875-8592 1574-0153 |
| Popis: | BACKGROUND Abnormal methylation of urinary TWIST1 and NID2 conferred high sensitivity and specificity for the detection of urothelial carcinoma. OBJECTIVE We examine the performance of the urine-based TWIST1/NID2 methylation assay with the addition of urine cytology for the detection of urothelial carcinoma. MATERIALS AND METHODS A prospective multi-institutional study was conducted to assess the performance of a methylation assay for patients with hematuria or under surveillance for non-muscle invasive bladder cancer (NMIBC). All patients underwent cystoscopy, a methylation assay, and cytology. Receiver operator characteristic (ROC) curves were constructed for cytology alone, the methylation assay alone, and a combined model. Areas under the curve (AUC) were compared using likelihood ratio tests. RESULTS A total of 172 patients were enrolled (37% for hematuria and 63% NMIBC). The AUC for cytology alone with equivocal cytologies positive was 0.704, and improved to 0.773 with the addition of the DNA methylation assay (p < 0.001). When the equivocal cytologies were considered negative, the AUC improved from 0.558 to 0.697 with the addition of the DNA methylation assay (p = 0.003). CONCLUSIONS Addition of a TWIST1/NID2-based DNA methylation assay adds diagnostic value to urine cytology and the model is sensitive to the classification of equivocal cytology. |
| Databáze: | OpenAIRE |
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