High-throughput manual parallel synthesis using SynPhase crowns and lanterns

Autor: Eric C. Bigham, David H. Drewry, Frank Navas, Michael J. Bishop, Stephen J. Hodson, Brian Edward Marron, Suganthini S. Nanthakumar, Judd Berman, Deanna T. Garrison, Lee Allen Barger, Mark H. Norman, Jennifer A. Kakel, Dennis Heyer, James A. Linn, Samuel W. Gerritz
Rok vydání: 2003
Předmět:
Zdroj: Journal of combinatorial chemistry. 5(2)
ISSN: 1520-4766
Popis: The high-throughput manual solid-phase parallel synthesis of libraries comprising thousands of discrete samples using pellicular supports (i.e. SynPhase crowns and lanterns) and a suite of novel tools and techniques is described. Key aspects of this approach include the combination of a split-split-split synthesis strategy with spatial encoding to differentiate thousands of crowns, the rapid washing and filtration of up to 48 reaction vessels in parallel, the application of an inexpensive and environmentally friendly technique to remove trifluoroacetic acid from sixteen 96-well plates in parallel, and a high-throughput method for removing cleaved crowns from reusable pin racks. Tens of thousands of discrete samples have been produced in-house using this conceptually and operationally straightforward strategy.
Databáze: OpenAIRE