Lactacystin inhibits 3T3-L1 adipocyte differentiation through induction of CHOP-10 expression
| Autor: | Qi Qun Tang, Hou Yan Song, Chun Gu Ma, Lin Jiang, Hong Lei Liu, Hai Yan Huang, Tanjing Song, De Guo Liu, Qun He, Jie Gen Chen, Xi Li, Duan Ma |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Lactacystin
Biophysics Mitosis Peroxisome proliferator-activated receptor Biology Biochemistry Mice chemistry.chemical_compound Downregulation and upregulation Transcription (biology) 3T3-L1 Cells Adipocyte Adipocytes medicine Animals Receptor Molecular Biology chemistry.chemical_classification CCAAT-Enhancer-Binding Protein-beta Cyclin-Dependent Kinase 2 Cell Differentiation DNA Cell Biology Phenotype Molecular biology Acetylcysteine Up-Regulation chemistry Proteasome inhibitor biological phenomena cell phenomena and immunity Transcription Factor CHOP Protein Binding medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 350:1-6 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2006.08.188 |
| Popis: | Hormonal induction triggers a cascade leading to the expression of CCAAT/enhancer-binding protein(C/EBP)alpha and peroxisome proliferator-activated receptor (PPAR) gamma, C/EBPalpha, and PPARgamma turns on series of adipocyte genes that give rise to the adipocyte phenotype. Previous findings indicate that C/EBPbeta, a transcriptional activator of the C/EBPalpha and PPARgamma genes, is rapidly expressed after induction, but lacks DNA-binding activity and therefore cannot activate transcription of the C/EBPalpha and PPARgamma genes early in the differentiation program. Acquisition of DNA-binding activity of C/EBPbeta occurs when CHOP-10, a dominant-negative form of C/EBP family members, is down-regulated and becomes hyperphosphorylated as preadipocytes traverse the G1-S checkpoint of mitotic clonal expansion. Evidences are presented in this report that lactacystin, a proteasome inhibitor, up-regulated the CHOP-10 expression, blocked the DNA-binding activity of C/EBPbeta, and subsequently inhibited MCE as well as adipocyte differentiation. |
| Databáze: | OpenAIRE |
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