| Autor: |
John A. Hamilton, T J Gonda, Gino Vairo, E J Cragoe, A M Bordun, S Argyriou |
| Rok vydání: |
1990 |
| Předmět: |
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| Zdroj: |
Scopus-Elsevier |
| ISSN: |
0021-9258 |
| DOI: |
10.1016/s0021-9258(17)44850-2 |
| Popis: |
Na+/H+ exchange activation by growth factors is proposed to be an important early signal for mitogenesis; however, little is known of its duration and requirement during later stages of the cell cycle. Macrophage-specific colony factor (CSF-1) rapidly activates murine bone marrow-derived macrophage Na+/H+ exchange, resulting in stimulation of Na+,K(+)-ATPase activity. The response to CSF-1 is maintained for at least 24 h. Inhibition of Na+/H+ exchange with 5-N,N-dimethylamiloride prevents CSF-1-stimulated DNA synthesis and cell growth. This is unlikely to be due to cytoplasmic acidosis, but more likely reflects a requirement for Na+/H+ exchange-mediated Na+ influx. DMA addition even up to 8 h after the growth factors suppresses S-phase progression. Na+/H+ exchange appears not to be involved in the induction of other early growth factor responses (c-fos and c-myc mRNA induction and general RNA and protein synthesis). We propose that growth factor-stimulated Na+/H+ exchange late in G1 of the cell cycle is required for S-phase progression but not for certain early growth factor responses. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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