The Protein-tyrosine Phosphatase SHP-2 Is Required during Angiotensin II-mediated Activation of Cyclin D1 Promoter in CHO-AT1A Cells
| Autor: | Arlette Levy, Bernard Rothhut, Laurent Guillemot, Zhizhuang Joe Zhao, Gilbert Béréziat |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Transcriptional Activation
MAPK/ERK pathway SH2 Domain-Containing Protein Tyrosine Phosphatases Cyclin D Cyclin A Protein Tyrosine Phosphatase Non-Receptor Type 11 CHO Cells Biology Biochemistry Catalysis Receptor Angiotensin Type 1 S Phase Proto-Oncogene Proteins p21(ras) src Homology Domains Phosphatidylinositol 3-Kinases chemistry.chemical_compound Cyclin D1 Cricetinae Animals Humans Promoter Regions Genetic Molecular Biology Protein Tyrosine Phosphatase Non-Receptor Type 1 Receptors Angiotensin Angiotensin II Protein Tyrosine Phosphatase Non-Receptor Type 6 G1 Phase Intracellular Signaling Peptides and Proteins Tyrosine phosphorylation Cell Biology Molecular biology Rats Proto-Oncogene Proteins c-raf Gene Expression Regulation chemistry biology.protein Cyclin-dependent kinase complex Mitogen-Activated Protein Kinases Protein Tyrosine Phosphatases Cyclin A2 |
| Zdroj: | Journal of Biological Chemistry. 275:26349-26358 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m001614200 |
| Popis: | Angiotensin II (Ang II) binds to specific G protein-coupled receptors and is mitogenic in Chinese hamster ovary (CHO) cells stably expressing a rat vascular angiotensin II type 1A receptor (CHO-AT(1A)). Cyclin D1 protein expression is regulated by mitogens, and its assembly with the cyclin-dependent kinases induces phosphorylation of the retinoblastoma protein pRb, a critical step in G(1) to S phase cell cycle progression contributing to the proliferative responses. In the present study, we found that in CHO-AT(1A) cells, Ang II induced a rapid and reversible tyrosine phosphorylation of various intracellular proteins including the protein-tyrosine phosphatase SHP-2. Ang II also induced cyclin D1 protein expression in a phosphatidylinositol 3-kinase and mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK)-dependent manner. Using a pharmacological and a co-transfection approach, we found that p21(ras), Raf-1, phosphatidylinositol 3-kinase and also the catalytic activity of SHP-2 and its Src homology 2 domains are required for cyclin D1 promoter/reporter gene activation by Ang II through the regulation of MAPK/ERK activity. Our findings suggest for the first time that SHP-2 could play an important role in the regulation of a gene involved in the control of cell cycle progression resulting from stimulation of a G protein-coupled receptor independently of epidermal growth factor receptor transactivation. |
| Databáze: | OpenAIRE |
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