Two calcium-binding chaperones from the fat body of the Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae) involved in diapause
| Autor: | Sabine Hänniger, Cathy Coutu, Cansu Doğan, Linda K. Crubaugh, Russell L. Groves, Dwayne D. Hegedus, David G. Heckel, Şerife Bayram, Umut Toprak |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Male Physiology Calnexin Fat Body Genes Insect Biology Diapause Diapause Insect 01 natural sciences Biochemistry 03 medical and health sciences Downregulation and upregulation Heat shock protein Animals Leptinotarsa Phylogeny Sex Characteristics Colorado potato beetle General Medicine biology.organism_classification Cell biology Coleoptera 010602 entomology 030104 developmental biology Starvation Insect Science Chaperone (protein) biology.protein Calcium Female Starvation response Calreticulin Molecular Chaperones |
| Zdroj: | Archives of insect biochemistry and physiologyREFERENCES. 106(1) |
| ISSN: | 1520-6327 |
| Popis: | Molecular chaperones are crucial for the correct folding of newly synthesized polypeptides, in particular, under stress conditions. Various studies have revealed the involvement of molecular chaperones, such as heat shock proteins, in diapause maintenance and starvation; however, the role of other chaperones in diapause and starvation relatively is unknown. In the current study, we identified two lectin-type chaperones with calcium affinity, a calreticulin (LdCrT) and a calnexin (LdCnX), that were present in the fat body of the Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae) during diapause. Both proteins possessed an N-globular domain, a P-arm domain, and a highly charged C-terminal domain, while an additional transmembrane domain was present in LdCnX. Phylogenetic analysis revealed distinction at the order level. Both genes were expressed in multiple tissues in larval and adult stages, and constitutively throughout development, though a starvation response was detected only for LdCrT. In females, diapause-related expression analysis in the whole body revealed an upregulation of both genes by post-diapause, but a downregulation by diapause only for LdCrT. By contrast, males revealed no alteration in their diapause-related expression pattern in the entire body for both genes. Fat body-specific expression analysis of both genes in relation to diapause revealed the same expression pattern with no alteration in females and downregulation in males by post-diapause. This study suggests that calcium-binding chaperones play similar and possibly gender-specific roles during diapause. |
| Databáze: | OpenAIRE |
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