Effect of Producer Cell Line on Functional Activity of Anti-D Monoclonal Antibodies Destined for Prevention of Rhesus Sensitization
| Autor: | G. Yu. Miterev, Olovnikova Ni, M. A. Ershler, T. L. Nikolaeva, Belkina Ev |
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| Rok vydání: | 2009 |
| Předmět: |
Erythrocytes
medicine.drug_class Rho(D) Immune Globulin Fc receptor Rh Isoimmunization Monoclonal antibody Monocytes General Biochemistry Genetics and Molecular Biology Immunoglobulin G Cell Line Mice Antigen Isoantibodies Cell Line Tumor Immune Tolerance medicine Animals Humans Lymphocytes Receptor B-Lymphocytes Rh-Hr Blood-Group System biology Receptors IgG Antibody-Dependent Cell Cytotoxicity Antibodies Monoclonal General Medicine Molecular biology Primary and secondary antibodies Rats Monoclonal biology.protein Antibody |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 147:448-452 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/s10517-009-0516-0 |
| Popis: | The ability of anti-D antibodies to cause antigen-specific immunosuppression depends on their interaction with low-affinity Fcgamma-receptors. Human monoclonal antibodies to D antigen of the rhesus system were investigated by antibody-dependent cytotoxicity assay in order to estimate their ability to induce hemolysis mediated by low-affinity Fcgamma receptors. We demonstrate that affinity of monoclonal antibodies to receptors of this type does not depend on primary structure of Fc-fragment, but depends on the producer cell line which expresses the antibodies. Monoclonal IgG1 antibodies interacting with FcgammaRIIa and FcgammaRIII lost this property, if they were secreted by human-mouse heterohybridoma, but not by human B-cell line. On the opposite, monoclonal antibodies that could not activate low-affinity Fcgamma receptors were highly active after human cells fusion with rat myeloma YB2/0. Hemolytic activity of IgG3 remained unchanged after fusion of human cells with rodent cells. |
| Databáze: | OpenAIRE |
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