Effects of hypoxia on differentiation of menstrual blood stromal stem cells towards tenogenic cells in a co-culture system with Achilles tendon cells
| Autor: | Chaonan Wang, Tao Cheng, Xiaolei Zhang, Jianjun Hong, Yijing Zheng, Xuqi Hu, Yuemiao Chen, Yifei Zhou, Xuhao Zheng |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research Pathology medicine.medical_specialty Stromal cell Achilles tendinitis Andrology 03 medical and health sciences menstrual blood stromal stem cells 0302 clinical medicine Immunology and Microbiology (miscellaneous) Thrombospondin 4 medicine education education.field_of_study Achilles tendon biology hypoxia Tenascin C Scleraxis Mesenchymal stem cell Articles differentiation General Medicine medicine.disease co-culture Achilles tendon cell 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis biology.protein Stem cell |
| Zdroj: | Experimental and Therapeutic Medicine |
| ISSN: | 1792-1015 1792-0981 |
| DOI: | 10.3892/etm.2017.4383 |
| Popis: | Achilles tendons have a very poor capacity for intrinsic regeneration. The cell-based treatment strategy for Achilles tendinitis includes the application of mesenchymal stem cells (MSCs), which have high proliferative and multipotent differentiation ability, and is a promising approach. The aim of the present study was to explore the tenogenic potential of human menstrual blood stromal stem cells (MenSCs) in a co-culture system and to compare the tenogenic capability under normoxic and hypoxic conditions. MenSCs were co-cultured indirectly with Achilles tendon cells in a Transwell co-culture system for 1, 2, or 3 weeks in two different concentrations of oxygen (20 and 2% O2), whereas the control contained only MenSCs. The extracellular matrix of MenSCs in each system was evaluated by Alcian blue staining assay, histological staining, reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and western blot analysis. Alcian blue staining assay revealed a significant increase (P |
| Databáze: | OpenAIRE |
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