Neutrophil migration induced by IL-8-activated mast cells is mediated by CINC-1
| Autor: | C. D. L. Ramos, N. E. Heluy-Neto, Fernando Q. Cunha, Ronaldo A. Ribeiro, S H Ferreira |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Chemokine Indoles Neutrophils Chemokine CXCL1 Immunology Indomethacin Biochemistry Dexamethasone Peritoneal cavity medicine Immunology and Allergy Animals Interleukin 8 Mast Cells Rats Wistar Molecular Biology Peritoneal Cavity biology Chemotactic Factors Chemistry Interleukin-8 Chemotaxis Hematology Neutrophil extracellular traps In vitro Cell biology Rats Thalidomide Interleukin 33 Chemotaxis Leukocyte medicine.anatomical_structure biology.protein Intercellular Signaling Peptides and Proteins Antibody Chemokines Chemokines CXC |
| Zdroj: | Cytokine. 21(5) |
| ISSN: | 1043-4666 |
| Popis: | The aim of this study was to characterize the mediators released by mast cells responsible for IL-8-induced neutrophil migration. It was observed that IL-8 induces a dose-dependent neutrophil migration into peritoneal cavity of rats, but not into air-pouch cavity in which resident mast cells are not present. The transference of peritoneal mast cells to the air-pouch renders this cavity responsive to IL-8. The neutrophil migration induced by IL-8 into the peritoneal cavity was not observed when the peritoneal-resident mast cells were depleted by compound 48/80 or distilled water treatment. Confirming the importance of mast cells, IL-8-stimulated mast cells supernatant induced significant neutrophil migration when injected into peritoneal and air-pouch cavities. The IL-8-induced neutrophil migration was observed not to be dependent on LTB(4), prostaglandins or TNF-alpha, since MK886, indomethacin or thalidomide were unable to block the IL-8-induced neutrophil accumulation 'in vivo' or the release of neutrophil chemotactic factor "in vitro" by IL-8-stimulated mast cells. However, dexamethasone, an inhibitor of the synthesis of pro-inflammatory cytokines, blocked the neutrophil migration induced by IL-8 "in vivo" and also inhibited the release of the neutrophil chemotactic factor by IL-8-stimulated mast cells. Moreover, the incubation of IL-8-stimulated mast cells supernatant with antibody against cytokine-induced neutrophil chemoattractant 1 (CINC-1), but not against TNF-alpha or IL-1beta, inhibited its neutrophil chemotactic activity. Furthermore, we found a significant amount of CINC-1 in this supernatant. In conclusion, we demonstrated that the neutrophil migration induced by IL-8 is dependent on CINC-1 release from mast cells. |
| Databáze: | OpenAIRE |
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