Genesis of unusual vesicles in rat periportal hepatocytes after administration of N-hydroxy-2-acetylaminofluorene
| Autor: | Geny M. M. Groothuis, Machiel J. Hardonk, Caesar E. Hulstaert, Dharamdajal Kalicharan |
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| Přispěvatelé: | Electron Microscopy, Biopharmaceuticals, Discovery, Design and Delivery (BDDD) |
| Rok vydání: | 1984 |
| Předmět: |
Male
Biology Endoplasmic Reticulum chemistry.chemical_compound Nucleotidases Animals 5'-Nucleotidase Carcinogen Adenosine Triphosphatases chemistry.chemical_classification Histocytochemistry Enzyme histochemistry Endoplasmic reticulum Vesicle Hydroxyacetylaminofluorene Rats Inbred Strains 2-Acetylaminofluorene Catalase Rats Enzyme Liver Biochemistry chemistry Glucose-6-Phosphatase Ultrastructure Premalignant lesion |
| Zdroj: | Virchows Archiv. Abteilung B: Cell Pathology, 46(1-2), 77-82 |
| ISSN: | 0042-6431 0340-6075 |
| DOI: | 10.1007/bf02890297 |
| Popis: | Administration of N-hydroxy-2-acetylaminofluorene (90 mumol/kg, iv) to rats results in damage to periportal hepatocytes. The most prominent ultrastructural changes are the appearance of numerous unusual vesicles of about 200 nm diameter and the proliferation of the endoplasmic reticulum to form fingerprint-like structures. In order to characterize these vesicles and to investigate their possible origin, their enzymatic activity was studied by ultrastructural enzyme histochemistry. The vesicles as well as the fingerprint-like structures exhibited glucose-6-phosphatase activity. Continuities between the vesicles and the membranes of both the RER and the SER were frequently seen. The vesicles lacked ATPase, 5'nucleotidase and catalase activity. From these results we conclude that the vesicles may be an unusual proliferation of the endoplasmic reticulum. |
| Databáze: | OpenAIRE |
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