Developmental stage-specific A-to-I editing pattern in the postnatal pineal gland of pigs (Sus scrofa)
| Autor: | Kui Li, Yalan Yang, Wen-Ye Yao, Chun-Di Xie, Hua Li, Yangli Pei, Zheng Feng, Rong Zhou, Zhang Leixia |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
RNA editing MiRNA binding Retrotransposon Biology Biochemistry 03 medical and health sciences Pineal gland Gene expression medicine Gene lcsh:SF1-1100 Pig lcsh:Veterinary medicine Research 0402 animal and dairy science RNA 04 agricultural and veterinary sciences 040201 dairy & animal science Cell biology Postnatal development 030104 developmental biology medicine.anatomical_structure ADAR lcsh:SF600-1100 Animal Science and Zoology lcsh:Animal culture A-to-I Food Science Biotechnology |
| Zdroj: | Journal of Animal Science and Biotechnology, Vol 11, Iss 1, Pp 1-9 (2020) Journal of Animal Science and Biotechnology |
| ISSN: | 2049-1891 |
| Popis: | Background RNA editing is a widespread post-transcriptional modification mechanism in mammalian genomes. Although many editing sites have been identified in domestic pigs (Sus scrofa), little is known about the characteristics and dynamic regulation of RNA editing in the pineal gland (PG), a small neuroendocrine gland that synthesizes and secretes melatonin, which is primarily responsible to modulate sleep patterns. Results This study analyzed the expression of adenosine-to-inosine (A-to-I) editing regulators and profiled the first dynamic A-to-I RNA editome during postnatal PG development. The results identified ADAR1 as the most abundantly expressed ADAR enzyme, which was down-regulated during postnatal PG development. Furthermore, 47,284 high-confidence RNA editing sites were identified, the majority of which (93.6%) were of the canonical A-to-I editing type, followed by C-to-T editing. Analysis of its characteristics showed that the A-to-I editing sites mostly localized in SINE retrotransposons PRE-1/Pre0_SS. Moreover, a strong deficiency and preference for guanine nucleotides at positions of one base upstream or downstream were found, respectively. The overall editing level at the puberty stage was higher than at both infancy and adulthood stages. Additionally, genome-wide RNA editing was found to exhibit a dynamic stage-specific fashion (postnatally). Genes that underwent developmental changes in RNA editing were associated with catabolic processes as well as protein localization and transport functions, implying that RNA editing might be responsible for the molecular machineries of the postnatal developing PG. Remarkably, RNA editing in 3′-UTRs might regulate gene expression by influencing miRNA binding during PG development. Conclusions This study profiles the first comprehensive developmental RNA editome in the pig PG, which contributes to the understanding of the importance of post-transcriptionally mediated regulation during mammalian postnatal PG development. Moreover, this study widely extends RNA editome resources in mammals. |
| Databáze: | OpenAIRE |
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